6smt

S-enantioselective imine reductase from Mycobacterium smegmatis

spinqualitylabelsall models 6smt, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6smt is a 5 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.55Å Ligands: 2EH, EDO, FMT, GOL, NDP, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0R5X0_MYCS2 Publication Abstract from PubMed NADPH-dependent imine reductases (IREDs) are enzymes capable of enantioselectively reducing imines to chiral secondary amines, which represent important building blocks in the chemical and pharmaceutical industry. Since their discovery in 2011, many previously unknown IREDs have been identified, biochemically and structurally characterized and categorized into families. However, the catalytic mechanism and guiding principles for substrate specificity and stereoselectivity remain disputed. Herein, we describe the crystal structure of S-IRED-Ms from Mycobacterium smegmatis together with its cofactor NADPH. S-IRED-Ms belongs to the S-enantioselective superfamily 3 (SFam3) and is the first IRED from SFam3 to be structurally described. The data presented provide further evidence for the overall high degree of structural conservation between different IREDs of various superfamilies. We discuss the role of Asp170 in catalysis and the importance of hydrophobic amino acids in the active site for stereospecificity. Moreover, a separate entrance to the active site, potentially functioning according to a gatekeeping mechanism regulating access and, therefore, substrate specificity is described. Structural Characterization of an S-enantioselective Imine Reductase from Mycobacterium Smegmatis.,Meyer T, Zumbragel N, Geerds C, Groger H, Niemann HH Biomolecules. 2020 Jul 31;10(8). pii: biom10081130. doi: 10.3390/biom10081130. PMID:32751900 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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