7cls: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Solution structure of an intramolecular G-quadruplex containing a duplex bulge== | |||
<StructureSection load='7cls' size='340' side='right'caption='[[7cls]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7cls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CLS FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cls OCA], [https://pdbe.org/7cls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cls RCSB], [https://www.ebi.ac.uk/pdbsum/7cls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cls ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Beyond the consensus definition of G-quadruplex-forming motifs with tracts of continuous guanines, G-quadruplexes harboring bulges in the G-tetrad core are prevalent in the human genome. Here, we study the incorporation of a duplex hairpin within a bulge of a G-quadruplex. The NMR solution structure of a G-quadruplex containing a duplex bulge was resolved, revealing the structural details of the junction between the duplex bulge and the G-quadruplex. Unexpectedly, instead of an orthogonal connection the duplex stem was observed to stack below the G-quadruplex forming a unique quadruplex-duplex junction. Breaking up of the immediate base pair step at the junction, coupled with a narrowing of the duplex groove within the context of the bulge, led to a progressive transition between the quadruplex and duplex segments. This study revealed that a duplex bulge can be formed at various positions of a G-quadruplex scaffold. In contrast to a non-structured bulge, the stability of a G-quadruplex slightly increases with an increase in the duplex bulge size. A G-quadruplex structure containing a duplex bulge of up to 33 nt in size was shown to form, which was much larger than the previously reported 7-nt bulge. With G-quadruplexes containing duplex bulges representing new structural motifs with potential biological significance, our findings would broaden the definition of potential G-quadruplex-forming sequences. | |||
Duplex formation in a G-quadruplex bulge.,Ngoc Nguyen TQ, Lim KW, Phan AT Nucleic Acids Res. 2020 Sep 22. pii: 5909921. doi: 10.1093/nar/gkaa738. PMID:32960213<ref>PMID:32960213</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7cls" style="background-color:#fffaf0;"></div> | ||
[[Category: Lim | == References == | ||
[[Category: Phan | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Lim KW]] | |||
[[Category: Nguyen TQN]] | |||
[[Category: Phan AT]] | |||
Latest revision as of 11:12, 14 June 2023
Solution structure of an intramolecular G-quadruplex containing a duplex bulge
| ||||||||||||
