1p7h: Difference between revisions

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New page: left|200px<br /> <applet load="1p7h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p7h, resolution 2.60Å" /> '''Structure of NFAT1 ...
 
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[[Image:1p7h.gif|left|200px]]<br />
<applet load="1p7h" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1p7h, resolution 2.60&Aring;" />
'''Structure of NFAT1 bound as a dimer to the HIV-1 LTR kB element'''<br />


==Overview==
==Structure of NFAT1 bound as a dimer to the HIV-1 LTR kB element==
DNA binding by NFAT1 as a dimer has been implicated in the activation of, host and viral genes. Here we report a crystal structure of NFAT1 bound, cooperatively as a dimer to the highly conserved kappa B site from the, human immunodeficiency virus 1 (HIV-1) long terminal repeat (LTR). This, structure reveals a new mode of dimerization and protein-DNA recognition, by the Rel homology region (RHR) of NFAT1. The two NFAT1 monomers form a, complete circle around the kappa B DNA through protein-protein, interactions mediated by both their N- and C-terminal subdomains. The, major dimer interface, formed by the C-terminal domain, is asymmetric and, substantially different from the symmetric dimer interface seen in other, Rel family proteins. Comparison to other NFAT structures, including NFAT5, and the NFAT1-Fos-Jun-ARRE2 complex, reveals that NFAT1 adopts different, conformations and its protein surfaces mediate distinct protein-protein, interactions in the context of different DNA sites.
<StructureSection load='1p7h' size='340' side='right'caption='[[1p7h]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1p7h]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P7H FirstGlance]. <br>
1P7H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P7H OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p7h OCA], [https://pdbe.org/1p7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p7h RCSB], [https://www.ebi.ac.uk/pdbsum/1p7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p7h ProSAT]</span></td></tr>
==Reference==
</table>
Structure of NFAT1 bound as a dimer to the HIV-1 LTR kappa B element., Giffin MJ, Stroud JC, Bates DL, von Koenig KD, Hardin J, Chen L, Nat Struct Biol. 2003 Oct;10(10):800-6. Epub 2003 Aug 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12949493 12949493]
== Function ==
[https://www.uniprot.org/uniprot/NFAC2_HUMAN NFAC2_HUMAN] Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.<ref>PMID:21871017</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/1p7h_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p7h ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bates, D.L.]]
[[Category: Bates DL]]
[[Category: Chen, L.]]
[[Category: Chen L]]
[[Category: Giffin, M.J.]]
[[Category: Giffin MJ]]
[[Category: Hardin, J.]]
[[Category: Hardin J]]
[[Category: Koenig, K.D.von.]]
[[Category: Stroud JC]]
[[Category: Stroud, J.C.]]
[[Category: Von Koenig KD]]
[[Category: activator]]
[[Category: dna binding protein]]
[[Category: transcription regulation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 14:22:56 2007''

Latest revision as of 08:06, 14 February 2024

Structure of NFAT1 bound as a dimer to the HIV-1 LTR kB element

1p7h, resolution 2.60Å

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