5ux0: Difference between revisions

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<StructureSection load='5ux0' size='340' side='right'caption='[[5ux0]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='5ux0' size='340' side='right'caption='[[5ux0]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5ux0]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UX0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5UX0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5ux0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinitoga_piezophila Marinitoga piezophila] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UX0 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ux0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ux0 OCA], [http://pdbe.org/5ux0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ux0 RCSB], [http://www.ebi.ac.uk/pdbsum/5ux0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ux0 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.197&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ux0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ux0 OCA], [https://pdbe.org/5ux0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ux0 RCSB], [https://www.ebi.ac.uk/pdbsum/5ux0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ux0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AGO_MARPK AGO_MARPK] An RNA-guided ssDNA endonuclease that may play a role in defense against invading mobile genetic elements. Uses short 5'-OH-ssRNA sequences as guides (gRNA) to bind complementary target DNA (tDNA) or target RNA resulting in target cleavage. The cleavage site is 10 nucleotides (nt) downstream of the target residue base-paired with the 5'-end of the gRNA. Reaction rates are fastest on 5'-OH-gRNA:tDNA followed by 5'-OH-gRNA:target RNA. gRNA between 17-21 nt supports equivalent rates of cleavage, has no preferred 5'-nt. Has weak activity on tDNA with 5'-phospho-gRNA, yielding products 1-2 nt longer (PubMed:27035975). Unlike other characterized prokaryotic Ago proteins symmetric mismatches centered around the cleavage site reduce cleavage efficiency (PubMed:28520746).<ref>PMID:27035975</ref> <ref>PMID:28520746</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Doudna, J A]]
[[Category: Marinitoga piezophila]]
[[Category: Doxzen, K W]]
[[Category: Synthetic construct]]
[[Category: Argonaute]]
[[Category: Doudna JA]]
[[Category: Dna]]
[[Category: Doxzen KW]]
[[Category: Hydrolase-rna-dna complex]]
[[Category: Rna]]
[[Category: Rnai]]
Retrieved from "https://proteopedia.org/5ux0"