7aba: Difference between revisions
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The | ==The structure of the Bottromycin biosynthetic protein SalCYP== | ||
<StructureSection load='7aba' size='340' side='right'caption='[[7aba]], [[Resolution|resolution]] 1.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7aba]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salinispora_tropica Salinispora tropica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ABA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ABA FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8500232Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aba OCA], [https://pdbe.org/7aba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aba RCSB], [https://www.ebi.ac.uk/pdbsum/7aba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aba ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bottromycins are ribosomally synthesized and post-translationally modified peptide natural product antibiotics that are effective against high-priority human pathogens such as methicillin-resistant Staphylococcus aureus. The total synthesis of bottromycins involves at least 17 steps, with a poor overall yield. Here, we report the characterization of the cytochrome P450 enzyme BotCYP from a bottromycin biosynthetic gene cluster. We determined the structure of a close BotCYP homolog and used our data to conduct the first large-scale survey of P450 enzymes associated with RiPP biosynthetic gene clusters. We demonstrate that BotCYP converts a C-terminal thiazoline to a thiazole via an oxidative decarboxylation reaction and provides stereochemical resolution for the pathway. Our data enable the two-pot in vitro production of the bottromycin core scaffold and may allow the rapid generation of bottromycin analogues for compound development. | |||
Characterization of the Stereoselective P450 Enzyme BotCYP Enables the In Vitro Biosynthesis of the Bottromycin Core Scaffold.,Adam S, Franz L, Milhim M, Bernhardt R, Kalinina OV, Koehnke J J Am Chem Soc. 2020 Dec 9;142(49):20560-20565. doi: 10.1021/jacs.0c10361. Epub, 2020 Nov 28. PMID:33249843<ref>PMID:33249843</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7aba" style="background-color:#fffaf0;"></div> | ||
[[Category: Adam | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Salinispora tropica]] | |||
[[Category: Adam S]] | |||
[[Category: Koehnke J]] | |||
Latest revision as of 06:11, 19 June 2024
The structure of the Bottromycin biosynthetic protein SalCYP
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