7k52: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 7k52 is ON HOLD  until Paper Publication
==Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III)==
<StructureSection load='7k52' size='340' side='right'caption='[[7k52]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7k52]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K52 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRO:PROLINE'>PRO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k52 OCA], [https://pdbe.org/7k52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k52 RCSB], [https://www.ebi.ac.uk/pdbsum/7k52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k52 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS9_ECOLI RS9_ECOLI] The C-terminal tail plays a role in the affinity of the 30S P site for different tRNAs. Mutations that decrease this affinity are suppressed in the 70S ribosome.<ref>PMID:15308780</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. How and where in the elongation cycle +1-frameshifting occurs remains poorly understood. We describe seven ~3.5-A-resolution cryo-EM structures of 70S ribosome complexes, allowing visualization of elongation and translocation by the GTPase elongation factor G (EF-G). Four structures with a + 1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G*GDPCP, the tRNA shifts to the +1-frame near the P site, rendering the freed mRNA base to bulge between the P and E sites and to stack on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during tRNA-mRNA translocation.


Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.
Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation.,Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA Nat Commun. 2021 Jul 30;12(1):4644. doi: 10.1038/s41467-021-24911-1. PMID:34330903<ref>PMID:34330903</ref>


Description: Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Demo, G]]
<div class="pdbe-citations 7k52" style="background-color:#fffaf0;"></div>
[[Category: Hou, Y.M]]
 
[[Category: Loveland, A.B]]
==See Also==
[[Category: Korostelev, A.A]]
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
[[Category: Gamper, H.B]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
[[Category: Svidritskiy, E]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Demo G]]
[[Category: Gamper HB]]
[[Category: Hou YM]]
[[Category: Korostelev AA]]
[[Category: Loveland AB]]
[[Category: Svidritskiy E]]
Retrieved from "https://proteopedia.org/7k52"