7kpl: Difference between revisions
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New page: '''Unreleased structure''' The entry 7kpl is ON HOLD Authors: Ahmed, M., Wang, P., Sadek, H. Description: Crystal structure of apo hEphB1 Category: Unreleased Structures [[Category... |
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==Crystal structure of hEphB1 in apo form== | |||
<StructureSection load='7kpl' size='340' side='right'caption='[[7kpl]], [[Resolution|resolution]] 2.71Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KPL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.705Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kpl OCA], [https://pdbe.org/7kpl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kpl RCSB], [https://www.ebi.ac.uk/pdbsum/7kpl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kpl ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Previous studies have demonstrated that the synaptic EphB1 receptor tyrosine kinase is a major mediator of neuropathic pain, suggesting that targeting the activity of this receptor might be a viable therapeutic option. Therefore, we set out to determine if any FDA-approved drugs can act as inhibitors of the EphB1 intracellular catalytic domain. An in silico screen was first used to identify a number of tetracycline antibiotics which demonstrated potential docking to the ATP-binding catalytic domain of EphB1. Kinase assays showed that demeclocycline, chlortetracycline, and minocycline inhibit EphB1 kinase activity at low micromolar concentrations. In addition, we cocrystallized chlortetracycline and EphB1 receptor, which confirmed its binding to the ATP-binding domain. Finally, in vivo administration of the three-tetracycline combination inhibited the phosphorylation of EphB1 in the brain, spinal cord, and dorsal root ganglion (DRG) and effectively blocked neuropathic pain in mice. These results indicate that demeclocycline, chlortetracycline, and minocycline can be repurposed for treatment of neuropathic pain and potentially for other indications that would benefit from inhibition of EphB1 receptor kinase activity. | |||
Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain.,Ahmed MS, Wang P, Nguyen NUN, Nakada Y, Menendez-Montes I, Ismail M, Bachoo R, Henkemeyer M, Sadek HA, Kandil ES Proc Natl Acad Sci U S A. 2021 Mar 9;118(10). pii: 2016265118. doi:, 10.1073/pnas.2016265118. PMID:33627480<ref>PMID:33627480</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Ahmed | <div class="pdbe-citations 7kpl" style="background-color:#fffaf0;"></div> | ||
[[Category: Sadek | |||
[[Category: Wang | ==See Also== | ||
*[[Ephrin receptor 3D structures|Ephrin receptor 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Ahmed M]] | |||
[[Category: Sadek H]] | |||
[[Category: Wang P]] | |||
Latest revision as of 11:32, 30 October 2024
Crystal structure of hEphB1 in apo form
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