Terminase: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
Michal Harel (talk | contribs)
32,811 edits
No edit summary
Michal Harel (talk | contribs)
32,811 edits
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>
<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>


 
__TOC__
== Function ==
== Function ==


'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses.  The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>.  The nuclease activity of the large Ter subunit is stimulated by ATP.  The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>.  '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.
'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses.  The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>.  The nuclease activity of the large Ter subunit is stimulated by ATP.  The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits ('''TRM1, TRM2 and TRM3'''), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>.  '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.


== Structural highlights ==
== Structural highlights ==


The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
== 3D Structures of terminase ==
[[Terminase 3D Structures ]]
</StructureSection>
</StructureSection>
== 3D Structures of terminase ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
*Terminase small subunit
**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
**[[6ey7]] – HvTer nuclease domain + inhibitor<br />
**[[3hef]] – Ter – Bacteriophage T7 <br />
**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />
**[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br />
**[[3zqp]], [[3zqm]], [[3zqn]], [[3zqo]] – Sf6Ter oligomerization domain  <br />
**[[3zqq]], [[4dyq]], [[4dyr]] – Sf6Ter  <br />
**[[1j9i]] – Ter nuclease domain – Bacteriophage λ - NMR <br />
**[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br />
**[[3p9a]] – Ter – Bacteriophage P22 <br />
**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />
**[[6ejq]] – Ter – Thermus phage<br />
**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ  <br />
**[[7joq]] – Ter – Pseudomonas  phage NV1<br />
**[[6w7t]] – Ter – Pseudomonas  phage PaP3<br />
*Terminase large subunit
**[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br />
**[[4bij]], [[4bil]] – T7Ter – Bacteriophage T7 – Cryo EM<br />
**[[4idh]] – T7Ter <br />
**[[2wbn]], [[2wc9]] – Ter nuclease domain – Bacteriophage Spp1 <br />
**[[3c6a]], [[3c6h]] – Ter nuclease domain – Bacteriophage Rb49 <br />
**[[5c10]], [[5c12]] – Sf6Ter nuclease domain <br />
**[[5c2d]], [[5c15]] – Sf6Ter nuclease domain (mutant)<br />
**[[5m1f]], [[5m1k]], [[5m1n]], [[5m1o]], [[5m1p]], [[5m1q]] – G20cTer  <br />
**[[5oe8]], [[5oee]], [[5oe9]] – D6eTer – Bacteriophage D6e <br />
**[[5tge]] – P74Ter nuclease domain – Thermus phage P74 <br />
**[[4zni]], [[4znk]] – P74Ter ATPase domain  <br />
**[[4znj]] – P74Ter ATPase domain (mutant)<br />
**[[4znl]] – P74Ter ATPase domain + ADP <br />
*Terminase large subunit complex
**[[4iee]], [[4ife]] – Sf6Ter + ATP<br />
**[[4iei]] – Sf6Ter + ADP<br />
**[[5c2f]] – Sf6Ter nuclease domain (mutant) + antifungal drug<br />
**[[5oea]] – D6eTer + ATP <br />
**[[5oeb]] – D6eTer + ADP <br />
**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />
*Tripartite terminase
**[[6m5s]], [[6m5r]] – HvTer TRM1+TRM2+TRM3 – Cryo EM<br />
**[[6m5u]], [[6m5v]] – HvTer TRM1+TRM2+TRM3 + ADP – Cryo EM<br />
**[[6m5t]] – HvTer TRM3 – Cryo EM<br />
}}


== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 07:52, 18 August 2024

Terminase large subunit complex with ATP (PDB code 4ife)

Drag the structure with the mouse to rotate

References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

Retrieved from "https://proteopedia.org/index.php?title=Terminase&oldid=4202234"