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| | ==STRUCTURAL VARIATION IN D(CTCTAGAG). IMPLICATIONS FOR PROTEIN-DNA INTERACTIONS== |
| The line below this paragraph, containing "STRUCTURE_1d93", creates the "Structure Box" on the page.
| | <StructureSection load='1d93' size='340' side='right'caption='[[1d93]], [[Resolution|resolution]] 2.15Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1d93]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D93 FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| -->
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d93 OCA], [https://pdbe.org/1d93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d93 RCSB], [https://www.ebi.ac.uk/pdbsum/1d93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d93 ProSAT]</span></td></tr> |
| {{STRUCTURE_1d93| PDB=1d93 | SCENE= }}
| | </table> |
| | | __TOC__ |
| '''STRUCTURAL VARIATION IN D(CTCTAGAG). IMPLICATIONS FOR PROTEIN-DNA INTERACTIONS'''
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| | | [[Category: Hunter WN]] |
| ==Overview== | | [[Category: Kennard O]] |
| Single-crystal X-ray diffraction techniques have been used to characterize the structure of the self-complementary DNA oligomer d(CTCTAGAG). The structure was refined to an R factor of 14.7% using data to 2.15-A resolution. The tetragonal unit cell, space group P4(3)2(1)2, has dimensions a = 42.53 and c = 24.33 A. The asymmetric unit consists of a single strand or four base pairs. Two strands, related by a crystallographic dyad axis, coil about each other to form a right-handed duplex. This octamer duplex has a mean helix rotation of 32 degrees, 11.3 base pairs per turn, an average rise of 3.1 A, C3'-endo furanose conformations, a shallow minor groove, and a deep major groove. Such averaged parameters suggest classification of the octamer as a member of the A-DNA family. However, the global parameters tend to mask variations in conformational parameters observed at the level of the base pairs. In particular, the central TpA (= TpA) step displays extensive interstrand purine-purine overlap and an unusual sugar-phosphate backbone conformation. These structural features may be directly related to certain sequence-specific protein-DNA interactions involving nucleases and repressors.
| | [[Category: Langlois D'Estaintot B]] |
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| ==About this Structure== | |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D93 OCA]. | |
| | |
| ==Reference== | |
| Structural variation in d(CTCTAGAG). Implications for protein-DNA interactions., Hunter WN, D'Estaintot BL, Kennard O, Biochemistry. 1989 Mar 21;28(6):2444-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2730875 2730875]
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| [[Category: Estaintot, B Langlois D.]] | |
| [[Category: Hunter, W N.]] | |
| [[Category: Kennard, O.]] | |
| [[Category: A-dna]] | |
| [[Category: Double helix]] | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:35:19 2008''
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