7bcv: Difference between revisions

Latest revision as of 08:54, 14 July 2024

Brevibacterium linens encapsulin structure

Structural highlights

7bcv is a 1 chain structure with sequence from Brevibacterium linens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 2.28Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENCAP_BRELN Shell component of a type 1 encapsulin nanocompartment. Assembles into proteinaceous shells 23-24 nm in diameter with 2-2.5 nm thick walls. Endogenous cargo protein DyP (dye-decolorizing peroxidase) is targeted to the interior via its C-terminal extension; only 1 DyP hexamer is incorporated into each shell. Empty shells can be isolated in the absence of cargo (PubMed:19172747, PubMed:27355101, PubMed:29166561). Cargo encapsulation probably precedes assembly of the nanocompartment; may assemble or disassemble via dimers, subcomplexes with a distinct preference for even numbers of subunits are detected (Probable) (PubMed:27355101). Nanocompartments are stable against mechanical forces; loaded nanocompartments are less stable than empty ones (PubMed:27355101). Nanocompartments are stable between pH 5-10; they aggregate at pH 9-10 and start to disassemble at pH 11. They are stable in 1M NaCl, 1 M MgCl(2) and 1M CaCl(2), unstable in 20% DMSO (dimethylsulfoxide) and are stable in 20% but not 40% ethanol (PubMed:29166561).^[1] ^[2] ^[3] ^[4]

References

↑ Sutter M, Boehringer D, Gutmann S, Gunther S, Prangishvili D, Loessner MJ, Stetter KO, Weber-Ban E, Ban N. Structural basis of enzyme encapsulation into a bacterial nanocompartment. Nat Struct Mol Biol. 2008 Sep;15(9):939-47. PMID:19172747
↑ Snijder J, Kononova O, Barbu IM, Uetrecht C, Rurup WF, Burnley RJ, Koay MS, Cornelissen JJ, Roos WH, Barsegov V, Wuite GJ, Heck AJ. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin. Biomacromolecules. 2016 Aug 8;17(8):2522-9. PMID:27355101 doi:10.1021/acs.biomac.6b00469
↑ Putri RM, Allende-Ballestero C, Luque D, Klem R, Rousou KA, Liu A, Traulsen CH, Rurup WF, Koay MST, Castón JR, Cornelissen JJLM. Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake. ACS Nano. 2017 Dec 26;11(12):12796-12804. PMID:29166561 doi:10.1021/acsnano.7b07669
↑ Snijder J, Kononova O, Barbu IM, Uetrecht C, Rurup WF, Burnley RJ, Koay MS, Cornelissen JJ, Roos WH, Barsegov V, Wuite GJ, Heck AJ. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin. Biomacromolecules. 2016 Aug 8;17(8):2522-9. PMID:27355101 doi:10.1021/acs.biomac.6b00469

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Sutter M, Boehringer D, Gutmann S, Gunther S, Prangishvili D, Loessner MJ, Stetter KO, Weber-Ban E, Ban N. Structural basis of enzyme encapsulation into a bacterial nanocompartment. Nat Struct Mol Biol. 2008 Sep;15(9):939-47. PMID:19172747

[2] Snijder J, Kononova O, Barbu IM, Uetrecht C, Rurup WF, Burnley RJ, Koay MS, Cornelissen JJ, Roos WH, Barsegov V, Wuite GJ, Heck AJ. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin. Biomacromolecules. 2016 Aug 8;17(8):2522-9. PMID:27355101 doi:10.1021/acs.biomac.6b00469

[3] Putri RM, Allende-Ballestero C, Luque D, Klem R, Rousou KA, Liu A, Traulsen CH, Rurup WF, Koay MST, Castón JR, Cornelissen JJLM. Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake. ACS Nano. 2017 Dec 26;11(12):12796-12804. PMID:29166561 doi:10.1021/acsnano.7b07669

[4] Snijder J, Kononova O, Barbu IM, Uetrecht C, Rurup WF, Burnley RJ, Koay MS, Cornelissen JJ, Roos WH, Barsegov V, Wuite GJ, Heck AJ. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin. Biomacromolecules. 2016 Aug 8;17(8):2522-9. PMID:27355101 doi:10.1021/acs.biomac.6b00469

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 7bcv is ON HOLD
+==Brevibacterium linens encapsulin structure==
+<StructureSection load='7bcv' size='340' side='right'caption='[[7bcv]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7bcv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacterium_linens Brevibacterium linens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BCV FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.28&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bcv OCA], [https://pdbe.org/7bcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bcv RCSB], [https://www.ebi.ac.uk/pdbsum/7bcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bcv ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ENCAP_BRELN ENCAP_BRELN] Shell component of a type 1 encapsulin nanocompartment. Assembles into proteinaceous shells 23-24 nm in diameter with 2-2.5 nm thick walls. Endogenous cargo protein DyP (dye-decolorizing peroxidase) is targeted to the interior via its C-terminal extension; only 1 DyP hexamer is incorporated into each shell. Empty shells can be isolated in the absence of cargo (PubMed:19172747, PubMed:27355101, PubMed:29166561). Cargo encapsulation probably precedes assembly of the nanocompartment; may assemble or disassemble via dimers, subcomplexes with a distinct preference for even numbers of subunits are detected (Probable) (PubMed:27355101). Nanocompartments are stable against mechanical forces; loaded nanocompartments are less stable than empty ones (PubMed:27355101). Nanocompartments are stable between pH 5-10; they aggregate at pH 9-10 and start to disassemble at pH 11. They are stable in 1M NaCl, 1 M MgCl(2) and 1M CaCl(2), unstable in 20% DMSO (dimethylsulfoxide) and are stable in 20% but not 40% ethanol (PubMed:29166561).<ref>PMID:19172747</ref> <ref>PMID:27355101</ref> <ref>PMID:29166561</ref> <ref>PMID:27355101</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Brevibacterium linens]]
+[[Category: Large Structures]]
+[[Category: Allende-Ballestero C]]
+[[Category: Caston JR]]
+[[Category: Cornelissen JJLM]]
+[[Category: Klem R]]
+[[Category: Luque D]]

7bcv: Difference between revisions

Latest revision as of 08:54, 14 July 2024

Brevibacterium linens encapsulin structure

Structural highlights

Function

References

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