7awz: Difference between revisions
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==Crystal structure of the computationally designed Scone-E protein== | ==Crystal structure of the computationally designed Scone-E protein== | ||
<StructureSection load='7awz' size='340' side='right'caption='[[7awz]]' scene=''> | <StructureSection load='7awz' size='340' side='right'caption='[[7awz]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWZ OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[7awz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWZ FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awz OCA], [https://pdbe.org/7awz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awz RCSB], [https://www.ebi.ac.uk/pdbsum/7awz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awz ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric beta-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives. | |||
Crystal structures of Scone: pseudosymmetric folding of a symmetric designer protein.,Mylemans B, Killian T, Vandebroek L, Van Meervelt L, Tame JRH, Parac-Vogt TN, Voet ARD Acta Crystallogr D Struct Biol. 2021 Jul 1;77(Pt 7):933-942. doi:, 10.1107/S2059798321005787. Epub 2021 Jun 18. PMID:34196619<ref>PMID:34196619</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7awz" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Synthetic construct]] | |||
[[Category: Mylemans B]] | [[Category: Mylemans B]] | ||
[[Category: Voet ARD]] | [[Category: Voet ARD]] | ||
