Replication protein E1: Difference between revisions

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New page: <StructureSection load='2ayg' size='340' side='right' caption='Human papillomavirus 6a regulatory protein E2 complex with DNA (PDB code 2ayg)' scene=''> ==Function== '''Replication p...
 
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<StructureSection load='2ayg' size='340' side='right' caption='Human papillomavirus 6a regulatory protein E2 complex with DNA (PDB code [[2ayg]])' scene=''>
<StructureSection load='1ksx' size='340' side='right' caption='Bovine papillomavirus 1 replication protein E1 complex with DNA (PDB code [[1ksx]])' scene='87/873777/Cv/1'>


==Function==
==Function==


'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication.  RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2).  The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis,  The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>.  RPE2 is found also in bovine papillomavirus (BPV).
'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication.  RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2).  The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis,  The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>.  RPE1 is found also in bovine papillomavirus (BPV).


== Disease ==
== Disease ==


RPE2 induces apoptosis of its host cells<ref>PMID:26906543</ref>.
RPE1 has been shown to mitigate host cell defenses leading to the development of cancer induces apoptosis of its host cells<ref>PMID:29495005</ref>.
 
==Relevance==
 
The detection of RPE1 mRNA and promoter methylation may serve as cancer prognostic marker.


== Structural highlights ==
== Structural highlights ==
   
   
The complex between HPV6a DBD and 18-bp DNA (PDB code [[2ayg]]) shows the binding of 2 recognition helices of the dimer to 2 successive major grooves of the target DNA making numerous contacts to the DNA backbone phosphate atoms and to its bases<ref>PMID:16914454</ref>.
The complex between BPV1 DBD and 21-bp DNA (PDB code [[1ksx]]) shows 2 DBD-DNA binding modules: a <scene name='87/873777/Cv/2'>binding loop</scene> and a <scene name='87/873777/Cv/4'>binding helix</scene> each interacting with a single strand. The protein-DNA interactions are hydrophilic to phosphate atoms and van der Waals hydrophobic ones<ref>PMID:11889054</ref>.
</StructureSection>
</StructureSection>
==3D structures of regulatory protein E2==
==3D structures of replication protein E1==


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}


Domains: transactivation (TAD) 2-201; DNA-binding (DBD) 286-365
Domains: helicase 301-605; DNA-binding (DBD) 286-365


[[1r8h]], [[2aye]] - RPE2 DBD - HPV6a<br />
[[1r9w]] - HpvRPE1 DBD – Human papilloma virus 18<br />
[[2ayb]], [[2ayg]] - RPE2 DBD + DNA<br />
[[1tue]] - HpvRPE1 428-631 (mutant) + RPE2 TAD<br />
[[1r6k]], [[1r6n]] - RPE2 TAD + inhibitor - HPV11<br />
[[2gxa]] - DpvRPE1 helicase domain + DNA + ADP – Delta papilloma virus 4<br />
[[1dto]] - RPE2 TAD - HPV16<br />
[[5a9k]] - BpvRPE1 helicase domain – Bovine papilloma virus - Cryo EM<br />
[[2nnu]] - RPE2 TAD + BRD4 peptide<br />
[[2v9p]] - BpvRPE1 helicase domain <br />
[[1by9]], [[2q79]] - RPE2 DBD<br />
[[1f08]] - BpvRPE1 DBD<br />
[[3mi7]] - RPE2 DBD (mutant)<br />
[[1ksx]], [[1ksy]] - BpvRPE1 DBD + DNA<br />
[[1r8p]], [[1zzf]] - RPE2 DBD - NMR<br />
[[7apd]] - BpvRPE1 helicase domain + DNA – Cryo EM<br />
[[1tue]] - RPE1 428-631 (mutant) + RPE2 TAD - HPV18<br />
[[1qqh]] - RPE2 TAD<br />
[[1f9f]] - RPE2 DBD<br />
[[1jj4]] - RPE2 DBD + DNA<br />
[[1dhm]] - RPE2 DBD - HPV31 - NMR<br />
[[1a7g]] - RPE2 DBD - HPV32<br />
[[2jeu]], [[2jex]] - RPE2 TAD (mutant) - BPV1<br />
[[1jjh]], [[6bus]] - RPE2 DBD<br />
[[1dbd]] - RPE2 DBD - NMR<br />
[[2bop]] - RPE2 DBD + DNA<br />
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]
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