Replication protein E1: Difference between revisions
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<StructureSection load=' | <StructureSection load='1ksx' size='340' side='right' caption='Bovine papillomavirus 1 replication protein E1 complex with DNA (PDB code [[1ksx]])' scene='87/873777/Cv/1'> | ||
==Function== | ==Function== | ||
'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. | '''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. RPE1 is found also in bovine papillomavirus (BPV). | ||
== Disease == | == Disease == | ||
RPE1 has been shown to mitigate host cell defenses leading to the development of cancer induces apoptosis of its host cells<ref>PMID:29495005</ref>. | |||
==Relevance== | |||
The detection of RPE1 mRNA and promoter methylation may serve as cancer prognostic marker. | |||
== Structural highlights == | == Structural highlights == | ||
The complex between | The complex between BPV1 DBD and 21-bp DNA (PDB code [[1ksx]]) shows 2 DBD-DNA binding modules: a <scene name='87/873777/Cv/2'>binding loop</scene> and a <scene name='87/873777/Cv/4'>binding helix</scene> each interacting with a single strand. The protein-DNA interactions are hydrophilic to phosphate atoms and van der Waals hydrophobic ones<ref>PMID:11889054</ref>. | ||
</StructureSection> | </StructureSection> | ||
==3D structures of | ==3D structures of replication protein E1== | ||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
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Domains: helicase 301-605; DNA-binding (DBD) 286-365 | Domains: helicase 301-605; DNA-binding (DBD) 286-365 | ||
[[1r9w]] - | [[1r9w]] - HpvRPE1 DBD – Human papilloma virus 18<br /> | ||
[[1tue]] - | [[1tue]] - HpvRPE1 428-631 (mutant) + RPE2 TAD<br /> | ||
[[ | [[2gxa]] - DpvRPE1 helicase domain + DNA + ADP – Delta papilloma virus 4<br /> | ||
[[5a9k]] - | [[5a9k]] - BpvRPE1 helicase domain – Bovine papilloma virus - Cryo EM<br /> | ||
[[ | [[2v9p]] - BpvRPE1 helicase domain <br /> | ||
[[1f08]] - | [[1f08]] - BpvRPE1 DBD<br /> | ||
[[1ksx]], [[1ksy]] - | [[1ksx]], [[1ksy]] - BpvRPE1 DBD + DNA<br /> | ||
[[7apd]] - BpvRPE1 helicase domain + DNA – Cryo EM<br /> | |||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Latest revision as of 07:58, 13 February 2022
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3D structures of replication protein E1
Updated on 13-February-2022
Domains: helicase 301-605; DNA-binding (DBD) 286-365
1ksx - HpvRPE1 DBD – Human papilloma virus 18
1tue - HpvRPE1 428-631 (mutant) + RPE2 TAD
2gxa - DpvRPE1 helicase domain + DNA + ADP – Delta papilloma virus 4
5a9k - BpvRPE1 helicase domain – Bovine papilloma virus - Cryo EM
2v9p - BpvRPE1 helicase domain
1f08 - BpvRPE1 DBD
1ksx, 1ksy - BpvRPE1 DBD + DNA
7apd - BpvRPE1 helicase domain + DNA – Cryo EM
