7ecd: Difference between revisions
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New page: '''Unreleased structure''' The entry 7ecd is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Crystal structure of Tam41 from Firmicutes bacterium, complex with CTP-Mg== | |||
<StructureSection load='7ecd' size='340' side='right'caption='[[7ecd]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ECD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ECD FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ecd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ecd OCA], [https://pdbe.org/7ecd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ecd RCSB], [https://www.ebi.ac.uk/pdbsum/7ecd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ecd ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Translocator assembly and maintenance 41 (Tam41) catalyzes the synthesis of cytidine diphosphate diacylglycerol (CDP-DAG), which is a high-energy intermediate phospholipid critical for generating cardiolipin in mitochondria. Although Tam41 is present almost exclusively in eukaryotic cells, a Firmicutes bacterium contains the gene encoding Tam41-type CDP-DAG synthase (FbTam41). FbTam41 converted phosphatidic acid (PA) to CDP-DAG using a ternary complex mechanism in vitro. Additionally, FbTam41 functionally substituted yeast Tam41 in vivo. These results demonstrate that Tam41-type CDP-DAG synthase functions in some prokaryotic cells. We determined the crystal structure of FbTam41 lacking the C-terminal 18 residues in the cytidine triphosphate (CTP)-Mg2+ bound form at a resolution of 2.6 A. The crystal structure showed that FbTam41 contained a positively charged pocket that specifically accommodated CTP-Mg2+ and PA in close proximity. By using this structure, we constructed a model for the full-length structure of FbTam41 containing the last alpha-helix, which was missing in the crystal structure. Based on this model, we propose a molecular mechanism for CDP-DAG synthesis in bacterial cells and mitochondria. | |||
Crystal structure of Tam41 cytidine diphosphate diacylglycerol synthase from a Firmicutes bacterium.,Kimura K, Kawai F, Kubota-Kawai H, Watanabe Y, Tomii K, Kojima R, Hirata K, Yamamori Y, Endo T, Tamura Y J Biochem. 2021 Dec 29. pii: 6488380. doi: 10.1093/jb/mvab154. PMID:34964897<ref>PMID:34964897</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7ecd" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Kawai F]] | |||
[[Category: Kimura K]] | |||
[[Category: Kubota-Kawai H]] | |||
[[Category: Tamura Y]] | |||
[[Category: Watanabe Y]] | |||
Latest revision as of 19:33, 29 May 2024
Crystal structure of Tam41 from Firmicutes bacterium, complex with CTP-Mg
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