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| <StructureSection load='7ci3' size='340' side='right'caption='[[7ci3]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='7ci3' size='340' side='right'caption='[[7ci3]], [[Resolution|resolution]] 2.20Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[7ci3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CI3 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CI3 FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF7a, NS7a, orf7a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ci3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ci3 OCA], [https://pdbe.org/7ci3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ci3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ci3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ci3 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ci3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ci3 OCA], [https://pdbe.org/7ci3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ci3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ci3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ci3 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Dysregulated immune cell responses have been linked to the severity of coronavirus disease 2019 (COVID-19), but the specific viral factors of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were currently unknown. Herein, we reveal that the Immunoglobulin-like fold ectodomain of the viral protein SARS-CoV-2 ORF7a interacts with high efficiency to CD14(+) monocytes in human peripheral blood, compared to pathogenic protein SARS-CoV ORF7a. The crystal structure of SARS-CoV-2 ORF7a at 2.2 A resolution reveals three remarkable changes on the amphipathic side of the four-stranded beta-sheet, implying a potential functional interface of the viral protein. Importantly, SARS-CoV-2 ORF7a coincubation with CD14(+) monocytes ex vivo triggered a decrease in HLA-DR/DP/DQ expression levels and upregulated significant production of proinflammatory cytokines, including IL-6, IL-1beta, IL-8, and TNF-alpha. Our work demonstrates that SARS-CoV-2 ORF7a is an immunomodulating factor for immune cell binding and triggers dramatic inflammatory responses, providing promising therapeutic drug targets for pandemic COVID-19.
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| Structural insight reveals SARS-CoV-2 ORF7a as an immunomodulating factor for human CD14(+) monocytes.,Zhou Z, Huang C, Zhou Z, Huang Z, Su L, Kang S, Chen X, Chen Q, He S, Rong X, Xiao F, Chen J, Chen S iScience. 2021 Mar 19;24(3):102187. doi: 10.1016/j.isci.2021.102187. Epub 2021, Feb 12. PMID:33615195<ref>PMID:33615195</ref>
| | ==See Also== |
| | | *[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 7ci3" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: 2019-ncov]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Chen, S]] | | [[Category: Chen S]] |
| [[Category: Zhou, Z]] | | [[Category: Zhou Z]] |
| [[Category: Accessory protein]]
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| [[Category: Er membrane]]
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| [[Category: Ig-like fold]]
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| [[Category: Type-i transmembrane protein]]
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| [[Category: Viral protein]]
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