AlphaFold2 examples from CASP 14: Difference between revisions
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**length 146, aligned residues '''61, RMSD 7.8 Å''', Z=3.3, structural alignment 11 %id. | **length 146, aligned residues '''61, RMSD 7.8 Å''', Z=3.3, structural alignment 11 %id. | ||
Liisa Holm<ref name="dali2020" /><ref name="holmquote">Quoted with permission from Liisa Holm, March, 2021.</ref> stated: "T1037 has a homologous template in the PDB. The parent structure of T1037, phage RNA polymerase (6vr4, 2166 amino acids), is homologous to the RNAi polymerase from Neurospora crassa ( | Liisa Holm<ref name="dali2020" /><ref name="holmquote">Quoted with permission from Liisa Holm, March, 2021.</ref> stated: "T1037 has a homologous template in the PDB. The parent structure of T1037, phage RNA polymerase (6vr4, 2166 amino acids), is homologous to the RNAi polymerase from Neurospora crassa (2j7n chain A, 934 amino acids)<ref name="6vr4" />. Dali aligns them over 564 residues with an RMSD of 4.8 A. 115 residues of the common core are in the T1037 substructure. Several long insertions in T1037/6vr4 relative to 2j7n (chain A) form subdomains, which point outwards from the common core. Similar massive adaptation of the common core is seen, for example, in the glucosyltransferase 1 family<ref>PMID: 7729407</ref>." | ||
The [https://fatcat.godziklab.org/ FATCAT Server] reported that in order to superpose 150 residues (37% of 404) of T1037 with the closest structure in the PDB, 3 twists at hinges were required, after which an RMSD of 3.1 Å was achieved. For a 200-residue superposition (50% of 404), the best results after 3 twists had an RMSD of 5.4 Å. | The [https://fatcat.godziklab.org/ FATCAT Server] reported that in order to superpose 150 residues (37% of 404) of T1037 with the closest structure in the PDB, 3 twists at hinges were required, after which an RMSD of 3.1 Å was achieved. For a 200-residue superposition (50% of 404), the best results after 3 twists had an RMSD of 5.4 Å. | ||
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For comparison, CASP 14 reported GDT_TS 86.96 for the AlphaFold2 prediction, while the AS2TS server calculated GDT_TS 85.87 vs. 7jx6 chain A, and 88.32 vs. 7JTL chain A. (These results were corrected for 90/92 and 91/92 residues, respectively.) Thus, there appears to be some unidentified minor discrepancy between the GDT_TS calculations of CASP-14 vs. the method detailed at [[Calculating GDT_TS]]. | For comparison, CASP 14 reported GDT_TS 86.96 for the AlphaFold2 prediction, while the AS2TS server calculated GDT_TS 85.87 vs. 7jx6 chain A, and 88.32 vs. 7JTL chain A. (These results were corrected for 90/92 and 91/92 residues, respectively.) Thus, there appears to be some unidentified minor discrepancy between the GDT_TS calculations of CASP-14 vs. the method detailed at [[Calculating GDT_TS]]. | ||
==See Also== | |||
*[[AlphaFold/Index]], a list of pages in Proteopedia about Alphafold. | |||
==References & Notes== | ==References & Notes== | ||
<references /> | <references /> | ||
