2i9s: Difference between revisions

Latest revision as of 19:03, 29 May 2024

The solution structure of the core of mesoderm development (MESD).

Structural highlights

2i9s is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MESD_MOUSE Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Mesoderm development (MESD) is a 224 amino acid mouse protein that acts as a molecular chaperone for receptors of the low-density lipoprotein receptor (LDLR) family. By recording (15)N-HSQC-NMR spectra of six different MESD constructs, we could determine a highly structured core region corresponding to residues 104-177. Here we firstly present the solution structure of this highly conserved core of MESD. It shows a four-stranded anti-parallel beta-sheet and two alpha-helices situated on one side of the sheet. Although described in the literature as structurally homologues to ferredoxins, the connectivity of secondary structure elements is different in the MESD fold. A structural comparison to entries of the PDB reveals a frequent domain with low sequence homology annotated as HMA and P-II domains in Pfam.

The solution structure of the core of mesoderm development (MESD), a chaperone for members of the LDLR-family.,Kohler C, Andersen OM, Diehl A, Krause G, Schmieder P, Oschkinat H J Struct Funct Genomics. 2006 Dec;7(3-4):131-8. Epub 2007 Mar 7. PMID:17342452^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hsieh JC, Lee L, Zhang L, Wefer S, Brown K, DeRossi C, Wines ME, Rosenquist T, Holdener BC. Mesd encodes an LRP5/6 chaperone essential for specification of mouse embryonic polarity. Cell. 2003 Feb 7;112(3):355-67. PMID:12581525
↑ Chen J, Liu CC, Li Q, Nowak C, Bu G, Wang J. Two Structural and Functional Domains of MESD Required for Proper Folding and Trafficking of LRP5/6. Structure. 2011 Mar 9;19(3):313-23. PMID:21397183 doi:10.1016/j.str.2011.01.010
↑ Kohler C, Andersen OM, Diehl A, Krause G, Schmieder P, Oschkinat H. The solution structure of the core of mesoderm development (MESD), a chaperone for members of the LDLR-family. J Struct Funct Genomics. 2006 Dec;7(3-4):131-8. Epub 2007 Mar 7. PMID:17342452 doi:10.1007/s10969-007-9016-5

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Hsieh JC, Lee L, Zhang L, Wefer S, Brown K, DeRossi C, Wines ME, Rosenquist T, Holdener BC. Mesd encodes an LRP5/6 chaperone essential for specification of mouse embryonic polarity. Cell. 2003 Feb 7;112(3):355-67. PMID:12581525

[2] Chen J, Liu CC, Li Q, Nowak C, Bu G, Wang J. Two Structural and Functional Domains of MESD Required for Proper Folding and Trafficking of LRP5/6. Structure. 2011 Mar 9;19(3):313-23. PMID:21397183 doi:10.1016/j.str.2011.01.010

[3] Kohler C, Andersen OM, Diehl A, Krause G, Schmieder P, Oschkinat H. The solution structure of the core of mesoderm development (MESD), a chaperone for members of the LDLR-family. J Struct Funct Genomics. 2006 Dec;7(3-4):131-8. Epub 2007 Mar 7. PMID:17342452 doi:10.1007/s10969-007-9016-5

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==The solution structure of the core of mesoderm development (MESD).==
-<StructureSection load='2i9s' size='340' side='right'caption='[[2i9s]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2i9s' size='340' side='right'caption='[[2i9s]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2i9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I9S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2i9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I9S FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mesdc2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9s OCA], [https://pdbe.org/2i9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i9s RCSB], [https://www.ebi.ac.uk/pdbsum/2i9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i9s ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/MESD_MOUSE MESD_MOUSE]] Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.<ref>PMID:12581525</ref> <ref>PMID:21397183</ref>
+[https://www.uniprot.org/uniprot/MESD_MOUSE MESD_MOUSE] Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.<ref>PMID:12581525</ref> <ref>PMID:21397183</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Andersen, O]]
+[[Category: Andersen O]]
-[[Category: Diehl, A]]
+[[Category: Diehl A]]
-[[Category: Koehler, C]]
+[[Category: Koehler C]]
-[[Category: Krause, G]]
+[[Category: Krause G]]
-[[Category: Oschkinat, H]]
+[[Category: Oschkinat H]]
-[[Category: Schmieder, P]]
+[[Category: Schmieder P]]
-[[Category: Chaperone]]
-[[Category: Ferredoxin-like-fold]]

2i9s: Difference between revisions

Latest revision as of 19:03, 29 May 2024

The solution structure of the core of mesoderm development (MESD).

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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