7enc: Difference between revisions

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New page: '''Unreleased structure''' The entry 7enc is ON HOLD Authors: Chen, X., Qi, Y., Wang, X., Wu, Z., Yin, X., Li, J., Liu, W., Xu, Y. Description: hPIC-MED [[Category: Unreleased Structur...
 
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'''Unreleased structure'''


The entry 7enc is ON HOLD
==TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)==
<StructureSection load='7enc' size='340' side='right'caption='[[7enc]], [[Resolution|resolution]] 4.13&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7enc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ENC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ENC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.13&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7enc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7enc OCA], [https://pdbe.org/7enc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7enc RCSB], [https://www.ebi.ac.uk/pdbsum/7enc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7enc ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 1.3-megadalton transcription factor IID (TFIID) is required for preinitiation complex (PIC) assembly and RNA polymerase II (Pol II)-mediated transcription initiation on almost all genes. The 26-subunit Mediator stimulates transcription and cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the Pol II C-terminal domain (CTD). We determined the structures of human Mediator in the Tail module-extended (at near-atomic resolution) and Tail-bent conformations and structures of TFIID-based PIC-Mediator (76 polypeptides, ~4.1 megadaltons) in four distinct conformations. PIC-Mediator assembly induces concerted reorganization (Head-tilting and Middle-down) of Mediator and creates a Head-Middle sandwich, which stabilizes two CTD segments and brings CTD to CDK7 for phosphorylation; this suggests a CTD-gating mechanism favorable for phosphorylation. The TFIID-based PIC architecture modulates Mediator organization and TFIIH stabilization, underscoring the importance of TFIID in orchestrating PIC-Mediator assembly.


Authors: Chen, X., Qi, Y., Wang, X., Wu, Z., Yin, X., Li, J., Liu, W., Xu, Y.
Structures of the human Mediator and Mediator-bound preinitiation complex.,Chen X, Yin X, Li J, Wu Z, Qi Y, Wang X, Liu W, Xu Y Science. 2021 Jun 4;372(6546):eabg0635. doi: 10.1126/science.abg0635. Epub 2021 , May 6. PMID:33958484<ref>PMID:33958484</ref>


Description: hPIC-MED
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Liu, W]]
<div class="pdbe-citations 7enc" style="background-color:#fffaf0;"></div>
[[Category: Chen, X]]
 
[[Category: Li, J]]
==See Also==
[[Category: Wu, Z]]
*[[Mediator|Mediator]]
[[Category: Yin, X]]
*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
[[Category: Xu, Y]]
== References ==
[[Category: Wang, X]]
<references/>
[[Category: Qi, Y]]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Chen X]]
[[Category: Li J]]
[[Category: Liu W]]
[[Category: Qi Y]]
[[Category: Wang X]]
[[Category: Wu Z]]
[[Category: Xu Y]]
[[Category: Yin X]]
Retrieved from "https://proteopedia.org/7enc"