7cfk: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Structure of the CBS domain of the bacterial CNNM/CorC family Mg2+ transporter in complex with the novel inhibitor IGN95a== | ==Structure of the CBS domain of the bacterial CNNM/CorC family Mg2+ transporter in complex with the novel inhibitor IGN95a== | ||
<StructureSection load='7cfk' size='340' side='right'caption='[[7cfk]]' scene=''> | <StructureSection load='7cfk' size='340' side='right'caption='[[7cfk]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CFK FirstGlance]. <br> | <table><tr><td colspan='2'>[[7cfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_parvatiensis Thermus parvatiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CFK FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cfk OCA], [https://pdbe.org/7cfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cfk RCSB], [https://www.ebi.ac.uk/pdbsum/7cfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cfk ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FX6:(2S)-2-[(6-azanyl-9H-purin-8-yl)sulfanyl]butanoic+acid'>FX6</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cfk OCA], [https://pdbe.org/7cfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cfk RCSB], [https://www.ebi.ac.uk/pdbsum/7cfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cfk ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A109QFA5_9DEIN A0A109QFA5_9DEIN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The CorC/CNNM family of Na(+)-dependent Mg(2+) transporters is ubiquitously conserved from bacteria to humans. CorC, the bacterial CorC/CNNM family of proteins, is involved in resistance to antibiotic exposure and in the survival of pathogenic microorganisms in their host environment. The CorC/CNNM family proteins possess a cytoplasmic region containing the regulatory ATP-binding site. CorC and CNNM have attracted interest as therapeutic targets, whereas inhibitors targeting the ATP-binding site have not been identified. Here, we performed a virtual screening of CorC by targeting its ATP-binding site, identified a compound named IGN95a with inhibitory effects on ATP binding and Mg(2+) export, and determined the cytoplasmic domain structure in complex with IGN95a. Furthermore, a chemical cross-linking experiment indicated that with ATP bound to the cytoplasmic domain, the conformational equilibrium of CorC was shifted more toward the inward-facing state of the transmembrane domain. In contrast, IGN95a did not induce such a shift. | |||
Identification and mechanistic analysis of an inhibitor of the CorC Mg(2+) transporter.,Huang Y, Mu K, Teng X, Zhao Y, Funato Y, Miki H, Zhu W, Xu Z, Hattori M iScience. 2021 Mar 26;24(4):102370. doi: 10.1016/j.isci.2021.102370. eCollection , 2021 Apr 23. PMID:33912817<ref>PMID:33912817</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7cfk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Thermus parvatiensis]] | |||
[[Category: Hattori M]] | [[Category: Hattori M]] | ||
[[Category: Huang Y]] | [[Category: Huang Y]] | ||
[[Category: Jin F]] | [[Category: Jin F]] | ||
Latest revision as of 16:07, 29 November 2023
Structure of the CBS domain of the bacterial CNNM/CorC family Mg2+ transporter in complex with the novel inhibitor IGN95a
| ||||||||||||
