Sandbox Reserved 1677: Difference between revisions
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The Amino acid sequence highlight how the catalytic cysteine and residues of the NAD(H)-binding site are highly conserved with major variations in the substrate binding site which leads to functional differences in Aldehyde dehydrogenases. Amino acids in AldC in the oligomerization domain change the electrostatic surface charge and the surface topology. | The Amino acid sequence highlight how the catalytic cysteine and residues of the NAD(H)-binding site are highly conserved with major variations in the substrate binding site which leads to functional differences in Aldehyde dehydrogenases. Amino acids in AldC in the oligomerization domain change the electrostatic surface charge and the surface topology. | ||
<scene name='87/873239/Spacefill/2'>Space filling Model</scene> shows the hydrophilic(green) and hydrophobic (purple) | <scene name='87/873239/Spacefill/2'>Space filling Model</scene> shows the hydrophilic(green) and hydrophobic (purple). | ||
=== Other important features === | === Other important features === | ||
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<scene name='87/873239/Interactions/4'>Effects of interactions</scene> | <scene name='87/873239/Interactions/4'>Effects of interactions</scene> | ||
Glu 391 Mutation, loss of Glu 257 side chain removed the internal interaction that helps position the amine group of the nicotinamide and changes at this residue could change the orientation of the nicotinamide group for hydride transfer. | Glu 391 Mutation, loss of Glu 257 side chain removed the internal interaction that helps position the amine group of the nicotinamide and changes at this residue could change the orientation of the nicotinamide group for hydride transfer. | ||
Mutation of Glu 391 to alanine eliminates interaction of the carboxylate group to the nicotinamide ribose group, which again can be complemented by an aspartate. Local conformational changes impact the positioning of the nicoitamide ring to impact activity of this mutant protein. | |||
<scene name='87/873239/Cartoon_view/5'>Interactions</scene> between amino acids. This helps to understand how the polar amino acids | <scene name='87/873239/Cartoon_view/5'>Interactions</scene> between amino acids. This helps to understand how the polar amino acids | ||
