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[[Image:1edr.gif|left|200px]]


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==MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO6AATTCGCG) AT 1.6 ANGSTROM==
The line below this paragraph, containing "STRUCTURE_1edr", creates the "Structure Box" on the page.
<StructureSection load='1edr' size='340' side='right'caption='[[1edr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1edr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EDR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EDR FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A47:N6-METHOXY+ADENOSINE+5-MONOPHOSPHATE'>A47</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
{{STRUCTURE_1edr|  PDB=1edr  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1edr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1edr OCA], [https://pdbe.org/1edr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1edr RCSB], [https://www.ebi.ac.uk/pdbsum/1edr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1edr ProSAT]</span></td></tr>
 
</table>
'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO6AATTCGCG) AT 1.6 ANGSTROM'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Chatake T]]
In a previous paper, 2'-deoxy-N(6)-methoxyadenosine (mo(6)A) was shown to form a mismatch base-pair with 2'-deoxycytidine with a Watson-Crick-type geometry. To fully understand the structural basis of genetic mutations with damaged DNA, it is necessary to examine whether the methoxylated adenine residue still has the ability to form the regular Watson-Crick pairing with a thymine residue. Therefore, a DNA dodecamer with the sequence d(CGCGmo(6)AATTCGCG) has been synthesized and its crystal structure determined. The methoxylation has no significant effect on the overall DNA conformation, which is that of a standard B-form duplex. The methoxylated adenine moieties adopt the amino tautomer with an anti conformation around the C(6)-N(6) bond to the N(1) atom, and they form a Watson-Crick base-pair with thymine residues on the opposite strand, similar to an unmodified adenine residue. It is concluded that methoxylated adenine can present two alternate faces for base-pairing, thanks to the amino&lt;--&gt;imino tautomerism allowed by methoxylation. Based on this property, two gene transition routes are proposed.
[[Category: Hikima T]]
 
[[Category: Matsuda A]]
==About this Structure==
[[Category: Ono A]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EDR OCA].  
[[Category: Takenaka A]]
 
[[Category: Ueno Y]]
==Reference==
Crystallographic studies on damaged DNAs. II. N(6)-methoxyadenine can present two alternate faces for Watson-Crick base-pairing, leading to pyrimidine transition mutagenesis., Chatake T, Hikima T, Ono A, Ueno Y, Matsuda A, Takenaka A, J Mol Biol. 1999 Dec 17;294(5):1223-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10600380 10600380]
[[Category: Chatake, T.]]
[[Category: Hikima, T.]]
[[Category: Matsuda, A.]]
[[Category: Ono, A.]]
[[Category: Takenaka, A.]]
[[Category: Ueno, Y.]]
[[Category: B-dna]]
[[Category: Damaged dna]]
[[Category: Deoxyribonucleic acid]]
[[Category: Double helix]]
[[Category: Methoxyadenosine]]
[[Category: Modified nucleotide]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:58:24 2008''

Latest revision as of 07:01, 7 February 2024

MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO6AATTCGCG) AT 1.6 ANGSTROM

1edr, resolution 1.60Å

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