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==Solution structure of the tandem zinc finger domain of fission yeast Stc1==
==Solution structure of the tandem zinc finger domain of fission yeast Stc1==
<StructureSection load='2luy' size='340' side='right'caption='[[2luy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2luy' size='340' side='right'caption='[[2luy]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2luy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUY FirstGlance]. <br>
<table><tr><td colspan='2'>[[2luy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPBP8B7.28c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2luy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2luy OCA], [https://pdbe.org/2luy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2luy RCSB], [https://www.ebi.ac.uk/pdbsum/2luy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2luy ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2luy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2luy OCA], [https://pdbe.org/2luy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2luy RCSB], [https://www.ebi.ac.uk/pdbsum/2luy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2luy ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/YORS_SCHPO YORS_SCHPO]] Required for meiotic chromosome segregation.<ref>PMID:16169489</ref> 
[https://www.uniprot.org/uniprot/YORS_SCHPO YORS_SCHPO] Required for meiotic chromosome segregation.<ref>PMID:16169489</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Noncoding RNAs can modulate gene expression by directing modifications to histones that alter chromatin structure. In fission yeast, siRNAs produced via the RNAi pathway direct modifications associated with heterochromatin formation. siRNAs associate with the RNAi effector protein Argonaute 1 (Ago1), targeting the Ago1-containing RNA-induced transcriptional silencing (RITS) complex to homologous nascent transcripts. This promotes recruitment of the Clr4 complex (CLRC), which mediates methylation of histone H3 on lysine 9 (H3K9me) in cognate chromatin. A key question is how the RNAi and chromatin modification machineries are connected. Stc1 is a small protein recently shown to associate with both Ago1 and CLRC and to play a pivotal role in mediating the RNAi-dependent recruitment of CLRC to chromatin. To understand its mode of action, we have performed a detailed structural and functional analysis of the Stc1 protein. Our analyses reveal that the conserved N-terminal region of Stc1 represents an unusual tandem zinc finger domain, with similarities to common LIM domains but distinguished by a lack of preferred relative orientation of the two zinc fingers. We demonstrate that this tandem zinc finger domain is involved in binding Ago1, whereas the nonconserved C-terminal region mediates association with CLRC. These findings elucidate the molecular basis for the coupling of RNAi to chromatin modification in fission yeast.
 
Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification.,He C, Pillai SS, Taglini F, Li F, Ruan K, Zhang J, Wu J, Shi Y, Bayne EH Proc Natl Acad Sci U S A. 2013 Apr 23. PMID:23613586<ref>PMID:23613586</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2luy" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Fission yeast]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bayne, E]]
[[Category: Schizosaccharomyces pombe 972h-]]
[[Category: He, C]]
[[Category: Bayne E]]
[[Category: Shi, Y]]
[[Category: He C]]
[[Category: Wu, J]]
[[Category: Shi Y]]
[[Category: Dna binding protein]]
[[Category: Wu J]]
[[Category: Tandem zinc finger]]

Latest revision as of 06:57, 1 May 2024

Solution structure of the tandem zinc finger domain of fission yeast Stc1

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