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==3D NMR structure of the cytoplasmic rhodanese domain of the inner membrane protein YgaP from Escherichia coli==
==3D NMR structure of the cytoplasmic rhodanese domain of the inner membrane protein YgaP from Escherichia coli==
<StructureSection load='2moi' size='340' side='right'caption='[[2moi]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='2moi' size='340' side='right'caption='[[2moi]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2moi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MOI FirstGlance]. <br>
<table><tr><td colspan='2'>[[2moi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MOI FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2moj|2moj]], [[2mol|2mol]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b2668, JW2643, ygaP, ygaP b2668 JW2643 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2moi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2moi OCA], [https://pdbe.org/2moi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2moi RCSB], [https://www.ebi.ac.uk/pdbsum/2moi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2moi ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2moi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2moi OCA], [https://pdbe.org/2moi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2moi RCSB], [https://www.ebi.ac.uk/pdbsum/2moi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2moi ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/YGAP_ECOLI YGAP_ECOLI]
The solution NMR structure of the alpha-helical integral membrane protein YgaP from Escherichia coli in mixed DHPC-7/LMPG micelles is presented. In these micelles, YgaP forms a homo-dimer with the two transmembrane helices being the dimer interface, while the N-terminal cytoplasmic domain comprises a rhodanese fold in accordance to its sequence homology to the rhodanese family of sulfurtransferases. The enzymatic sulfur-transfer activity of full-length YgaP as well as of the N-terminal rhodanese domain only was investigated performing series of titrations with sodium thiosulfate and potassium cyanide monitored by NMR and EPR. The data indicate the thiosulfate concentration-dependent addition of several sulfur atoms to the catalytic Cys63, which process can be reversed by the addition of potassium cyanide. The catalytic reaction induces thereby conformational changes within the rhodanese domain, as well as on the transmembrane alpha-helices of YgaP. These results provide insights into a potential mechanism of YgaP during the catalytic thiosulfate activity in vivo.
 
Solution NMR Structure and Functional Analysis of the Integral Membrane Protein YgaP from E. coli.,Eichmann C, Tzitzilonis C, Bordignon E, Maslennikov I, Choe S, Riek R J Biol Chem. 2014 Jun 23. pii: jbc.M114.571935. PMID:24958726<ref>PMID:24958726</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2moi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ecoli]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bordignon, E]]
[[Category: Bordignon E]]
[[Category: Choe, S]]
[[Category: Choe S]]
[[Category: Eichmann, C]]
[[Category: Eichmann C]]
[[Category: Maslennikov, I]]
[[Category: Maslennikov I]]
[[Category: Riek, R]]
[[Category: Riek R]]
[[Category: Tzitzilonis, C]]
[[Category: Tzitzilonis C]]
[[Category: Membrane protein]]
[[Category: Rhodanese domain]]

Latest revision as of 07:01, 1 May 2024

3D NMR structure of the cytoplasmic rhodanese domain of the inner membrane protein YgaP from Escherichia coli

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