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==Solution structure of the fluorogen-activating protein FAST in complex with the ligand N871b==
==Solution structure of the fluorogen-activating protein FAST in complex with the ligand N871b==
<StructureSection load='7ava' size='340' side='right'caption='[[7ava]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='7ava' size='340' side='right'caption='[[7ava]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7ava]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AVA FirstGlance]. <br>
<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AVA FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=S1Q:(5~{Z})-5-[(2-methoxy-4-oxidanyl-phenyl)methylidene]-3-methyl-2-[(~{E})-2-pyridin-4-ylethenyl]imidazol-4-one'>S1Q</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=S1Q:(5~{Z})-5-[(2-methoxy-4-oxidanyl-phenyl)methylidene]-3-methyl-2-[(~{E})-2-pyridin-4-ylethenyl]imidazol-4-one'>S1Q</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ava FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ava OCA], [https://pdbe.org/7ava PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ava RCSB], [https://www.ebi.ac.uk/pdbsum/7ava PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ava ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ava FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ava OCA], [https://pdbe.org/7ava PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ava RCSB], [https://www.ebi.ac.uk/pdbsum/7ava PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ava ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
One of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids.
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.,Mineev KS, Goncharuk SA, Goncharuk MV, Povarova NV, Sokolov AI, Baleeva NS, Smirnov AY, Myasnyanko IN, Ruchkin DA, Bukhdruker S, Remeeva A, Mishin A, Borshchevskiy V, Gordeliy V, Arseniev AS, Gorbachev DA, Gavrikov AS, Mishin AS, Baranov MS Chem Sci. 2021 Apr 8;12(19):6719-6725. doi: 10.1039/d1sc01454d. PMID:34040747<ref>PMID:34040747</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ava" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Synthetic construct sequences]]
[[Category: Baranov MS]]
[[Category: Baranov, M S]]
[[Category: Goncharuk SA]]
[[Category: Goncharuk, S A]]
[[Category: Mineev KS]]
[[Category: Mineev, K S]]
[[Category: Fluorescent protein]]
[[Category: Fluorogen-activating protein pas domain]]

Latest revision as of 06:11, 19 June 2024

Solution structure of the fluorogen-activating protein FAST in complex with the ligand N871b

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