7r6m: Difference between revisions
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==Post-2S intermediate of the Tetrahymena group I intron, symmetry-expanded monomer from a synthetic dimeric construct== | |||
<StructureSection load='7r6m' size='340' side='right'caption='[[7r6m]], [[Resolution|resolution]] 3.68Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7r6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R6M FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.68Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r6m OCA], [https://pdbe.org/7r6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r6m RCSB], [https://www.ebi.ac.uk/pdbsum/7r6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r6m ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
High-resolution structural studies are essential for understanding the folding and function of diverse RNAs. Herein, we present a nanoarchitectural engineering strategy for efficient structural determination of RNA-only structures using single-particle cryogenic electron microscopy (cryo-EM). This strategy-ROCK (RNA oligomerization-enabled cryo-EM via installing kissing loops)-involves installing kissing-loop sequences onto the functionally nonessential stems of RNAs for homomeric self-assembly into closed rings with multiplied molecular weights and mitigated structural flexibility. ROCK enables cryo-EM reconstruction of the Tetrahymena group I intron at 2.98-A resolution overall (2.85 A for the core), allowing de novo model building of the complete RNA, including the previously unknown peripheral domains. ROCK is further applied to two smaller RNAs-the Azoarcus group I intron and the FMN riboswitch, revealing the conformational change of the former and the bound ligand in the latter. ROCK holds promise to greatly facilitate the use of cryo-EM in RNA structural studies. | |||
Sub-3-A cryo-EM structure of RNA enabled by engineered homomeric self-assembly.,Liu D, Thelot FA, Piccirilli JA, Liao M, Yin P Nat Methods. 2022 May 2. pii: 10.1038/s41592-022-01455-w. doi:, 10.1038/s41592-022-01455-w. PMID:35501384<ref>PMID:35501384</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7r6m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Tetrahymena thermophila]] | |||
[[Category: Liao M]] | |||
[[Category: Liu D]] | |||
[[Category: Thelot F]] | |||
[[Category: Yin P]] | |||
Latest revision as of 05:45, 5 June 2024
Post-2S intermediate of the Tetrahymena group I intron, symmetry-expanded monomer from a synthetic dimeric construct
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