2r5u: Difference between revisions

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<StructureSection load='2r5u' size='340' side='right'caption='[[2r5u]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='2r5u' size='340' side='right'caption='[[2r5u]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2r5u]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5U FirstGlance]. <br>
<table><tr><td colspan='2'>[[2r5u]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5U FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5u OCA], [https://pdbe.org/2r5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5u RCSB], [https://www.ebi.ac.uk/pdbsum/2r5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5u ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5u OCA], [https://pdbe.org/2r5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5u RCSB], [https://www.ebi.ac.uk/pdbsum/2r5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/DNAB_MYCTU DNAB_MYCTU]] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity (By similarity).  The intein is an endonuclease (Potential).  
[https://www.uniprot.org/uniprot/DNAB_MYCTU DNAB_MYCTU] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity (By similarity).  The intein is an endonuclease (Potential).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r5u ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r5u ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hexameric DnaB helicase unwinds the DNA double helix during replication of genetic material in bacteria. DnaB is an essential bacterial protein; therefore, it is an important potential target for antibacterial drug discovery. We report a crystal structure of the N-terminal region of DnaB from the pathogen Mycobacterium tuberculosis (MtDnaBn), determined at 2.0 A resolution. This structure provides atomic resolution details of formation of the hexameric ring of DnaB by two distinct interfaces. An extensive hydrophobic interface stabilizes a dimer of MtDnaBn by forming a four-helix bundle. The other, less extensive, interface is formed between the dimers, connecting three of them into a hexameric ring. On the basis of crystal packing interactions between MtDnaBn rings, we suggest a model of a helicase-primase complex that explains previously observed effects of DnaB mutations on DNA priming.
Hexameric ring structure of the N-terminal domain of Mycobacterium tuberculosis DnaB helicase.,Biswas T, Tsodikov OV FEBS J. 2008 Jun;275(12):3064-71. Epub 2008 May 8. PMID:18479467<ref>PMID:18479467</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2r5u" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Helicase 3D structures|Helicase 3D structures]]
*[[Helicase 3D structures|Helicase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Biswas, T]]
[[Category: Biswas T]]
[[Category: Tsodikov, O V]]
[[Category: Tsodikov OV]]
[[Category: Atp-binding]]
[[Category: Autocatalytic cleavage]]
[[Category: Dna replication]]
[[Category: Dna-binding]]
[[Category: Dnab]]
[[Category: Endonuclease]]
[[Category: Helicase]]
[[Category: Hydrolase]]
[[Category: Intron homing]]
[[Category: Nuclease]]
[[Category: Nucleotide-binding]]
[[Category: Primase]]
[[Category: Primosome]]
[[Category: Replication]]

Latest revision as of 09:20, 21 February 2024

Crystal structure of the N-terminal domain of DnaB helicase from Mycobacterium tuberculosis

2r5u, resolution 1.90Å

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