7rmf: Difference between revisions
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New page: '''Unreleased structure''' The entry 7rmf is ON HOLD Authors: Hansen, J.M., Lynch, E.M., Farrell, D.P., DiMaio, F., Quispe, J., Kollman, J.M. Description: Substrate-bound Ura7 filament... |
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The | ==Substrate-bound Ura7 filament at low pH== | ||
<StructureSection load='7rmf' size='340' side='right'caption='[[7rmf]], [[Resolution|resolution]] 7.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RMF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rmf OCA], [https://pdbe.org/7rmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rmf RCSB], [https://www.ebi.ac.uk/pdbsum/7rmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rmf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Many metabolic enzymes self-assemble into micron-scale filaments to organize and regulate metabolism. The appearance of these assemblies often coincides with large metabolic changes as in development, cancer, and stress. Yeast undergo cytoplasmic acidification upon starvation, triggering the assembly of many metabolic enzymes into filaments. However, it is unclear how these filaments assemble at the molecular level and what their role is in the yeast starvation response. CTP Synthase (CTPS) assembles into metabolic filaments across many species. Here, we characterize in vitro polymerization and investigate in vivo consequences of CTPS assembly in yeast. Cryo-EM structures reveal a pH-sensitive assembly mechanism and highly ordered filament bundles that stabilize an inactive state of the enzyme, features unique to yeast CTPS. Disruption of filaments in cells with non-assembly or pH-insensitive mutations decreases growth rate, reflecting the importance of regulated CTPS filament assembly in homeotstasis. | |||
Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.,Hansen JM, Horowitz A, Lynch EM, Farrell DP, Quispe J, DiMaio F, Kollman JM Elife. 2021 Nov 4;10. pii: 73368. doi: 10.7554/eLife.73368. PMID:34734801<ref>PMID:34734801</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7rmf" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Kollman | __TOC__ | ||
[[Category: Lynch | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: DiMaio F]] | |||
[[Category: Farrell DP]] | |||
[[Category: Hansen JM]] | |||
[[Category: Kollman JM]] | |||
[[Category: Lynch EM]] | |||
[[Category: Quispe J]] | |||
Latest revision as of 05:47, 5 June 2024
Substrate-bound Ura7 filament at low pH
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