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[[Image:1ezm.gif|left|200px]]
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{{STRUCTURE_1ezm|  PDB=1ezm  |  SCENE=  }}
'''THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION'''


==THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION==
<StructureSection load='1ezm' size='340' side='right'caption='[[1ezm]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ezm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EZM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ezm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezm OCA], [https://pdbe.org/1ezm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ezm RCSB], [https://www.ebi.ac.uk/pdbsum/1ezm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ezm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ELAS_PSEAE ELAS_PSEAE] Cleaves elastin, collagen, IgG, and several complement components. Involved in the pathogenesis of P.aeruginosa infections.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/1ezm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ezm ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pseudomonas aeruginosa elastase (PAE) is a zinc metalloprotease with 301 amino acids. We have crystallized and solved the three-dimensional structure of PAE, using data to 1.5-A resolution, and have refined the native molecular structure to R = 0.188. The overall tertiary structure of the PAE molecule is similar to that of thermolysin, with which it shares 28% amino acid sequence identity. Nearly all of the active site residues that might potentially interact with substrates are identical in the two proteins. However, the active site cleft is significantly more "open" in PAE than in thermolysin.


==Overview==
Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution.,Thayer MM, Flaherty KM, McKay DB J Biol Chem. 1991 Feb 15;266(5):2864-71. PMID:1899664<ref>PMID:1899664</ref>
Pseudomonas aeruginosa elastase (PAE) is a zinc metalloprotease with 301 amino acids. We have crystallized and solved the three-dimensional structure of PAE, using data to 1.5-A resolution, and have refined the native molecular structure to R = 0.188. The overall tertiary structure of the PAE molecule is similar to that of thermolysin, with which it shares 28% amino acid sequence identity. Nearly all of the active site residues that might potentially interact with substrates are identical in the two proteins. However, the active site cleft is significantly more "open" in PAE than in thermolysin.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1EZM is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZM OCA].
</div>
<div class="pdbe-citations 1ezm" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution., Thayer MM, Flaherty KM, McKay DB, J Biol Chem. 1991 Feb 15;266(5):2864-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1899664 1899664]
*[[Elastase 3D structures|Elastase 3D structures]]
[[Category: Pseudolysin]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Flaherty, K M.]]
__TOC__
[[Category: Mckay, D B.]]
</StructureSection>
[[Category: Thayer, M M.]]
[[Category: Large Structures]]
[[Category: Hydrolase]]
[[Category: Pseudomonas aeruginosa]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:42:40 2008''
[[Category: Flaherty KM]]
[[Category: Mckay DB]]
[[Category: Thayer MM]]
Retrieved from "https://proteopedia.org/1ezm"