7pdd: Difference between revisions
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New page: '''Unreleased structure''' The entry 7pdd is ON HOLD Authors: Qi, C., Korkhov, V.M. Description: Focus refinement of soluble domain of Adenylyl cyclase 9 in complex with DARPin C4 and ... |
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The | ==Focus refinement of soluble domain of Adenylyl cyclase 9 in complex with DARPin C4 and MANT-GTP== | ||
<StructureSection load='7pdd' size='340' side='right'caption='[[7pdd]], [[Resolution|resolution]] 4.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7pdd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PDD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PDD FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pdd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pdd OCA], [https://pdbe.org/7pdd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pdd RCSB], [https://www.ebi.ac.uk/pdbsum/7pdd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pdd ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/E1BM79_BOVIN E1BM79_BOVIN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The enzyme is weakly activated by forskolin, fully activated by the G protein Galphas subunit and is autoinhibited by the AC9 C-terminus. Although our recent structural studies of the AC9-Galphas complex provided the framework for understanding AC9 autoinhibition, the conformational changes that AC9 undergoes in response to activator binding remains poorly understood. Here, we present the cryo-EM structures of AC9 in several distinct states: (i) AC9 bound to a nucleotide inhibitor MANT-GTP, (ii) bound to an artificial activator (DARPin C4) and MANT-GTP, (iii) bound to DARPin C4 and a nucleotide analogue ATPalphaS, (iv) bound to Galphas and MANT-GTP. The artificial activator DARPin C4 partially activates AC9 by binding at a site that overlaps with the Galphas binding site. Together with the previously observed occluded and forskolin-bound conformations, structural comparisons of AC9 in the four conformations described here show that secondary structure rearrangements in the region surrounding the forskolin binding site are essential for AC9 activation. | |||
Structural basis of adenylyl cyclase 9 activation.,Qi C, Lavriha P, Mehta V, Khanppnavar B, Mohammed I, Li Y, Lazaratos M, Schaefer JV, Dreier B, Pluckthun A, Bondar AN, Dessauer CW, Korkhov VM Nat Commun. 2022 Feb 24;13(1):1045. doi: 10.1038/s41467-022-28685-y. PMID:35210418<ref>PMID:35210418</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Korkhov | <div class="pdbe-citations 7pdd" style="background-color:#fffaf0;"></div> | ||
[[Category: Qi | |||
==See Also== | |||
*[[3D Adenylyl cyclase 3D structures|3D Adenylyl cyclase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bos taurus]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Korkhov VM]] | |||
[[Category: Qi C]] | |||
Latest revision as of 12:31, 17 July 2024
Focus refinement of soluble domain of Adenylyl cyclase 9 in complex with DARPin C4 and MANT-GTP
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