1f2v: Difference between revisions

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[[Image:1f2v.jpg|left|200px]]


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==CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE OF AEROBIC VITAMIN B12 SYNTHESIS==
The line below this paragraph, containing "STRUCTURE_1f2v", creates the "Structure Box" on the page.
<StructureSection load='1f2v' size='340' side='right'caption='[[1f2v]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1f2v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_denitrificans_(nom._rej.) Pseudomonas denitrificans (nom. rej.)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F2V FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2v OCA], [https://pdbe.org/1f2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f2v RCSB], [https://www.ebi.ac.uk/pdbsum/1f2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2v ProSAT]</span></td></tr>
{{STRUCTURE_1f2v|  PDB=1f2v  |  SCENE= }}
</table>
 
== Function ==
'''CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE OF AEROBIC VITAMIN B12 SYNTHESIS'''
[https://www.uniprot.org/uniprot/COBH_SINSX COBH_SINSX] Catalyzes the conversion of precorrin-8X to hydrogenobyrinate.<ref>PMID:1732194</ref>
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
BACKGROUND: The crystal structure of precorrin-8x methyl mutase (CobH), an enzyme of the aerobic pathway to vitamin B12, provides evidence that the mechanism for methyl migration can plausibly be regarded as an allowed [1,5]-sigmatropic shift of a methyl group from C-11 to C-12 at the C ring of precorrin-8x to afford hydrogenobyrinic acid. RESULTS: The dimeric structure of CobH creates a set of shared active sites that readily discriminate between different tautomers of precorrin-8x and select a discrete tautomer for sigmatropic rearrangement. The active site contains a strictly conserved histidine residue close to the site of methyl migration in ring C of the substrate. CONCLUSION: Analysis of the structure with bound product suggests that the [1,5]-sigmatropic shift proceeds by protonation of the ring C nitrogen, leading to subsequent methyl migration.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/1f2v_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1F2V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_denitrificans Pseudomonas denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2V OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f2v ConSurf].
Crystal structure of precorrin-8x methyl mutase., Shipman LW, Li D, Roessner CA, Scott AI, Sacchettini JC, Structure. 2001 Jul 3;9(7):587-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11470433 11470433]
<div style="clear:both"></div>
[[Category: Precorrin-8X methylmutase]]
== References ==
[[Category: Pseudomonas denitrificans]]
<references/>
[[Category: Single protein]]
__TOC__
[[Category: Li, D.]]
</StructureSection>
[[Category: Roessner, C A.]]
[[Category: Large Structures]]
[[Category: Sacchettini, J C.]]
[[Category: Li D]]
[[Category: Scott, A I.]]
[[Category: Roessner CA]]
[[Category: Shipman, L W.]]
[[Category: Sacchettini JC]]
[[Category: Alpha-beta wind]]
[[Category: Scott AI]]
[[Category: Doubly wound sheet]]
[[Category: Shipman LW]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:49:33 2008''
Retrieved from "https://proteopedia.org/1f2v"