7pmd: Difference between revisions
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New page: '''Unreleased structure''' The entry 7pmd is ON HOLD Authors: Description: Category: Unreleased Structures |
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The | ==Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er)== | ||
<StructureSection load='7pmd' size='340' side='right'caption='[[7pmd]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7pmd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Amanita_phalloides Amanita phalloides], [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PMD FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=DTH:D-THREONINE'>DTH</scene>, <scene name='pdbligand=EEP:(2S,4R)-2-amino-4,5-dihydroxy-4-methylpentanoic+acid+(non-preferred+name)'>EEP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pmd OCA], [https://pdbe.org/7pmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pmd RCSB], [https://www.ebi.ac.uk/pdbsum/7pmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pmd ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding is only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin. | |||
High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.,Pospich S, Sweeney HL, Houdusse A, Raunser S Elife. 2021 Nov 23;10. pii: 73724. doi: 10.7554/eLife.73724. PMID:34812732<ref>PMID:34812732</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7pmd" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Actin 3D structures|Actin 3D structures]] | |||
*[[Myosin 3D Structures|Myosin 3D Structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Amanita phalloides]] | |||
[[Category: Gallus gallus]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Oryctolagus cuniculus]] | |||
[[Category: Houdusse A]] | |||
[[Category: Pospich S]] | |||
[[Category: Raunser S]] | |||
[[Category: Sweeney HL]] | |||
Latest revision as of 08:26, 4 March 2026
Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er)
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