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New page: left|200px<br /> <applet load="1csg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1csg, resolution 2.7Å" /> '''THREE-DIMENSIONAL ST...
 
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[[Image:1csg.gif|left|200px]]<br />
<applet load="1csg" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1csg, resolution 2.7&Aring;" />
'''THREE-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR'''<br />


==Overview==
==Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor==
The crystal structure of recombinant human granulocyte-macrophage, colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A, resolution using multiple isomorphous replacement techniques. There are, two molecules in the crystallographic asymmetric unit, which are related, by an approximate non-crystallographic 2-fold axis. The overall structure, is highly compact and globular with a predominantly hydrophobic core. The, main structural feature of rhGM-CSF is a four alpha-helix bundle, which, represents approximately 42% of the structure. The helices are arranged in, a left-handed antiparallel bundle with two overhand connections. Within, the connections is a two-stranded antiparallel beta-sheet. The tertiary, structure of rhGM-CSF has a topology similar to that of porcine growth, factor and interferon-beta. Most of the proposed critical regions for, receptor binding are located on a continuous surface at one end of the, molecule that includes the C terminus.
<StructureSection load='1csg' size='340' side='right'caption='[[1csg]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1csg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CSG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1csg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1csg OCA], [https://pdbe.org/1csg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1csg RCSB], [https://www.ebi.ac.uk/pdbsum/1csg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1csg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CSF2_HUMAN CSF2_HUMAN] Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cs/1csg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1csg ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus.


==Disease==
Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor.,Walter MR, Cook WJ, Ealick SE, Nagabhushan TL, Trotta PP, Bugg CE J Mol Biol. 1992 Apr 20;224(4):1075-85. PMID:1569568<ref>PMID:1569568</ref>
Known disease associated with this structure: Leukemia, acute myeloid, M2 type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=306250 306250]]


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1CSG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CSG OCA].
</div>
<div class="pdbe-citations 1csg" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor., Walter MR, Cook WJ, Ealick SE, Nagabhushan TL, Trotta PP, Bugg CE, J Mol Biol. 1992 Apr 20;224(4):1075-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1569568 1569568]
*[[Colony-stimulating factor 3D structures|Colony-stimulating factor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cook, W.J.]]
[[Category: Cook WJ]]
[[Category: Ealick, S.E.]]
[[Category: Ealick SE]]
[[Category: Walter, M.R.]]
[[Category: Walter MR]]
[[Category: cytokine]]
 
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