7qe3: Difference between revisions

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'''Unreleased structure'''


The entry 7qe3 is ON HOLD
==Se-M variant of B-trefoil lectin from Salpingoeca rosetta in complex with GalNAc==
<StructureSection load='7qe3' size='340' side='right'caption='[[7qe3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qe3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salpingoeca_rosetta Salpingoeca rosetta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QE3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1JW:(2~{S})-hexane-1,2,6-triol'>1JW</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qe3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qe3 OCA], [https://pdbe.org/7qe3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qe3 RCSB], [https://www.ebi.ac.uk/pdbsum/7qe3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qe3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F2UID9_SALR5 F2UID9_SALR5]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Choanoflagellates are primitive protozoa used as models for animal evolution. They express a large variety of multi-domain proteins contributing to adhesion and cell communication, thereby providing a rich repertoire of molecules for biotechnology. Adhesion often involves proteins adopting a beta-trefoil fold with carbohydrate-binding properties therefore classified as lectins. Sequence database screening with a dedicated method resulted in TrefLec, a database of 44714 beta-trefoil candidate lectins across 4497 species. TrefLec was searched for original domain combinations, which led to single out SaroL-1 in the choanoflagellate Salpingoeca rosetta, that contains both beta-trefoil and aerolysin-like pore-forming domains. Recombinant SaroL-1 is shown to bind galactose and derivatives, with a stronger affinity for cancer-related alpha-galactosylated epitopes such as the glycosphingolipid Gb3, when embedded in giant unilamellar vesicles or cell membranes. Crystal structures of complexes with Gb3 trisaccharide and GalNAc provided the basis for building a model of the oligomeric pore. Finally, recognition of the alphaGal epitope on glycolipids required for hemolysis of rabbit erythrocytes suggests that toxicity on cancer cells is achieved through carbohydrate-dependent pore-formation.


Authors: Notova, S., Varrot, A.
The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3.,Notova S, Bonnardel F, Rosato F, Siukstaite L, Schwaiger J, Lim JH, Bovin N, Varrot A, Ogawa Y, Romer W, Lisacek F, Imberty A Commun Biol. 2022 Sep 12;5(1):954. doi: 10.1038/s42003-022-03869-w. PMID:36097056<ref>PMID:36097056</ref>


Description: B-trefoil lectin from Salpingoeca rosetta in complex with GalNAc
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Varrot, A]]
<div class="pdbe-citations 7qe3" style="background-color:#fffaf0;"></div>
[[Category: Notova, S]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Salpingoeca rosetta]]
[[Category: Notova S]]
[[Category: Varrot A]]
Retrieved from "https://proteopedia.org/7qe3"