Pho4: Difference between revisions
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<StructureSection load='' size='400' side='right' caption='Yeast Pho4 DNA-binding domain complex with DNA (PDB code [[1a0a]]) ' scene='72/725407/Cv/1' pspeed='8'> | |||
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Phosphate system positive regulatory protein '''Pho4''' is a transcriptional activator which regulates the expression of phosphatase under conditions of phosphate starvation in Saccharomyces cerevisiae <ref>PMID:2220078</ref>. For details see [[Pho4 bHLH Protein]]. | Phosphate system positive regulatory protein '''Pho4''' is a transcriptional activator which regulates the expression of phosphatase under conditions of phosphate starvation in Saccharomyces cerevisiae <ref>PMID:2220078</ref>. For details see [[Pho4 bHLH Protein]]. | ||
*<scene name='72/725407/Cv/3'>Yeast Pho4 DNA-binding domain interactions with DNA</scene>. | *<scene name='72/725407/Cv/3'>Yeast Pho4 DNA-binding domain interactions with DNA</scene>. | ||
Its structure is a basic basic helix-loop-helix (bHLH) | Its structure is a basic basic helix-loop-helix (bHLH) | ||
The pho4 protein takes part in transcription of several yeast genes related to phosphate metabolism: | The pho4 protein takes part in transcription of several yeast genes related to phosphate metabolism: | ||
'''PH05 encoding repressible acid phosphatase,''' the expression of Pho5 in Saccharomyces cerevisiae is derepressed by a specific level of inorganic phosphate (Pi), and depressed when the Pi concentrate in the medium is lowered, the regulatory system of the Pi signals coded by PHO4 PHO2 , PHO80, and PHO81 genes.<ref>PMID:2671650</ref> | '''PH05 encoding repressible acid phosphatase,''' the expression of Pho5 in Saccharomyces cerevisiae is derepressed by a specific level of inorganic phosphate (Pi), and depressed when the Pi concentrate in the medium is lowered, the regulatory system of the Pi signals coded by PHO4 PHO2 , PHO80, and PHO81 genes.<ref>PMID:2671650</ref> | ||
'''PH08 encoding alkaline phosphatase''' | '''PH08 encoding alkaline phosphatase''' | ||
'''PH081 a regulatory gene''' regulated by PHO system wuth the use of Pi | '''PH081 a regulatory gene''' regulated by PHO system wuth the use of Pi | ||
'''PH084 phosphate permease gene'''<ref>PMID:3902805</ref> | '''PH084 phosphate permease gene'''<ref>PMID:3902805</ref> | ||
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*DNA binding | *DNA binding | ||
The Pho4 protein binds to DNA as a homodimer, and the binding region binds specifically to E-box sequence CACGTG and it's 3'-flanking bases by a specific subset of the basic region of the bHLH motif.<ref>PMID:1861859</ref> | The Pho4 protein binds to DNA as a homodimer, and the binding region binds specifically to E-box sequence CACGTG and it's 3'-flanking bases by a specific subset of the basic region of the bHLH motif.<ref>PMID:1861859</ref> | ||
the residues who takes part in the E-box recognition are His5, Glu9, and Arg13. | the residues who takes part in the E-box recognition are His5, Glu9, and Arg13. | ||
== 3D Structures of Pho == | == 3D Structures of Pho == | ||
