7qda: Difference between revisions
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The | ==Crystal structure of CalpL== | ||
<StructureSection load='7qda' size='340' side='right'caption='[[7qda]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7qda]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurihydrogenibium_sp._YO3AOP1 Sulfurihydrogenibium sp. YO3AOP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QDA FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qda OCA], [https://pdbe.org/7qda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qda RCSB], [https://www.ebi.ac.uk/pdbsum/7qda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qda ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CRISPR defence systems such as the well-known DNA-targeting Cas9 and the RNA-targeting type III systems are widespread in prokaryotes(1,2). The latter orchestrates a complex antiviral response that is initiated through the synthesis of cyclic oligoadenylates after recognition of foreign RNA(3-5). Among the large set of proteins that are linked to type III systems and predicted to bind cyclic oligoadenylates(6,7), a CRISPR-associated Lon protease (CalpL) stood out to us. CalpL contains a sensor domain of the SAVED family(7) fused to a Lon protease effector domain. However, the mode of action of this effector is unknown. Here we report the structure and function of CalpL and show that this soluble protein forms a stable tripartite complex with two other proteins, CalpT and CalpS, that are encoded on the same operon. After activation by cyclic tetra-adenylate (cA(4)), CalpL oligomerizes and specifically cleaves the MazF homologue CalpT, which releases the extracytoplasmic function sigma factor CalpS from the complex. Our data provide a direct connection between CRISPR-based detection of foreign nucleic acids and transcriptional regulation. Furthermore, the presence of a SAVED domain that binds cyclic tetra-adenylate in a CRISPR effector reveals a link to the cyclic-oligonucleotide-based antiphage signalling system. | |||
Antiviral signalling by a cyclic nucleotide activated CRISPR protease.,Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G Nature. 2023 Feb;614(7946):168-174. doi: 10.1038/s41586-022-05571-7. Epub 2022 , Nov 24. PMID:36423657<ref>PMID:36423657</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7qda" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sulfurihydrogenibium sp. YO3AOP1]] | |||
[[Category: Hagelueken G]] | |||
[[Category: Schneberger N]] | |||
