7qia: Difference between revisions
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The | ==Structure of apo-EleNRMT in complex with two nanobodies at 3.5A== | ||
<StructureSection load='7qia' size='340' side='right'caption='[[7qia]], [[Resolution|resolution]] 3.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7qia]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Eggerthella_lenta Eggerthella lenta] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIA FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qia OCA], [https://pdbe.org/7qia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qia RCSB], [https://www.ebi.ac.uk/pdbsum/7qia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qia ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/C8WJ67_EGGLE C8WJ67_EGGLE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca(2+) and Mg(2+), which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H(+) serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg(2+). The protein transports Mg(2+) and Mn(2+) but not Ca(2+) by a mechanism that is not coupled to H(+). Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell. | |||
Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family.,Ramanadane K, Straub MS, Dutzler R, Manatschal C Elife. 2022 Jan 10;11:e74589. doi: 10.7554/eLife.74589. PMID:35001872<ref>PMID:35001872</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7qia" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Dutzler | <references/> | ||
[[Category: Ramanadane | __TOC__ | ||
</StructureSection> | |||
[[Category: Eggerthella lenta]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Dutzler R]] | |||
[[Category: Manatschal C]] | |||
[[Category: Ramanadane K]] | |||
[[Category: Straub MS]] | |||
