7qin: Difference between revisions
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==In situ structure of actomyosin complex in skeletal sarcomere== | |||
<StructureSection load='7qin' size='340' side='right'caption='[[7qin]], [[Resolution|resolution]] 6.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7qin]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qin OCA], [https://pdbe.org/7qin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qin RCSB], [https://www.ebi.ac.uk/pdbsum/7qin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qin ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ACTS_MOUSE ACTS_MOUSE] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In skeletal muscle, nebulin stabilizes and regulates the length of thin filaments, but the underlying mechanism remains nebulous. In this work, we used cryo-electron tomography and subtomogram averaging to reveal structures of native nebulin bound to thin filaments within intact sarcomeres. This in situ reconstruction provided high-resolution details of the interaction between nebulin and actin, demonstrating the stabilizing role of nebulin. Myosin bound to the thin filaments exhibited different conformations of the neck domain, highlighting its inherent structural variability in muscle. Unexpectedly, nebulin did not interact with myosin or tropomyosin, but it did interact with a troponin T linker through two potential binding motifs on nebulin, explaining its regulatory role. Our structures support the role of nebulin as a thin filament "molecular ruler" and provide a molecular basis for studying nemaline myopathies. | |||
Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin.,Wang Z, Grange M, Pospich S, Wagner T, Kho AL, Gautel M, Raunser S Science. 2022 Feb 18;375(6582):eabn1934. doi: 10.1126/science.abn1934. Epub 2022 , Feb 18. PMID:35175800<ref>PMID:35175800</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7qin" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Actin 3D structures|Actin 3D structures]] | |||
*[[Myosin 3D Structures|Myosin 3D Structures]] | |||
*[[Nebulin|Nebulin]] | |||
*[[Tropomyosin 3D structures|Tropomyosin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | |||
[[Category: Gautel M]] | |||
[[Category: Grange M]] | |||
[[Category: Kho AL]] | |||
[[Category: Pospich S]] | |||
[[Category: Raunser S]] | |||
[[Category: Wagner T]] | |||
[[Category: Wang Z]] | |||
