7v93: Difference between revisions
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==Cryo-EM structure of the Cas12c2-sgRNA binary complex== | |||
<StructureSection load='7v93' size='340' side='right'caption='[[7v93]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7v93]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_archaeon Uncultured archaeon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V93 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v93 OCA], [https://pdbe.org/7v93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v93 RCSB], [https://www.ebi.ac.uk/pdbsum/7v93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v93 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA-guided CRISPR-Cas nucleases are widely used as versatile genome-engineering tools. Recent studies identified functionally divergent type V Cas12 family enzymes. Among them, Cas12c2 binds a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA) and recognizes double-stranded DNA targets with a short TN PAM. Here, we report the cryo-electron microscopy structures of the Cas12c2-guide RNA binary complex and the Cas12c2-guide RNA-target DNA ternary complex. The structures revealed that the crRNA and tracrRNA form an unexpected X-junction architecture, and that Cas12c2 recognizes a single T nucleotide in the PAM through specific hydrogen-bonding interactions with two arginine residues. Furthermore, our biochemical analyses indicated that Cas12c2 processes its precursor crRNA to a mature crRNA using the RuvC catalytic site through a unique mechanism. Collectively, our findings improve the mechanistic understanding of diverse type V CRISPR-Cas effectors. | |||
Structure of the type V-C CRISPR-Cas effector enzyme.,Kurihara N, Nakagawa R, Hirano H, Okazaki S, Tomita A, Kobayashi K, Kusakizako T, Nishizawa T, Yamashita K, Scott DA, Nishimasu H, Nureki O Mol Cell. 2022 May 19;82(10):1865-1877.e4. doi: 10.1016/j.molcel.2022.03.006. , Epub 2022 Apr 1. PMID:35366394<ref>PMID:35366394</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7v93" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Uncultured archaeon]] | |||
[[Category: Hirano H]] | |||
[[Category: Kobayashi K]] | |||
[[Category: Kurihara N]] | |||
[[Category: Kusakizako T]] | |||
[[Category: Nishimasu H]] | |||
[[Category: Nishizawa T]] | |||
[[Category: Nureki O]] | |||
[[Category: Tomita A]] | |||
[[Category: Yamashita K]] | |||
Latest revision as of 05:16, 12 June 2024
Cryo-EM structure of the Cas12c2-sgRNA binary complex
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