7qpg: Difference between revisions
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The | ==Human RZZ kinetochore corona complex.== | ||
<StructureSection load='7qpg' size='340' side='right'caption='[[7qpg]], [[Resolution|resolution]] 3.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7qpg]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QPG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qpg OCA], [https://pdbe.org/7qpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qpg RCSB], [https://www.ebi.ac.uk/pdbsum/7qpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qpg ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ZWILC_HUMAN ZWILC_HUMAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In metazoans, a approximately 1 megadalton (MDa) multiprotein complex comprising the dynein-dynactin adaptor Spindly and the ROD-Zwilch-ZW10 (RZZ) complex is the building block of a fibrous biopolymer, the kinetochore fibrous corona. The corona assembles on mitotic kinetochores to promote microtubule capture and spindle assembly checkpoint (SAC) signaling. We report here a high-resolution cryo-EM structure that captures the essential features of the RZZ complex, including a farnesyl-binding site required for Spindly binding. Using a highly predictive in vitro assay, we demonstrate that the SAC kinase MPS1 is necessary and sufficient for corona assembly at supercritical concentrations of the RZZ-Spindly (RZZS) complex, and describe the molecular mechanism of phosphorylation-dependent filament nucleation. We identify several structural requirements for RZZS polymerization in rings and sheets. Finally, we identify determinants of kinetochore localization and corona assembly of Spindly. Our results describe a framework for the long-sought-for molecular basis of corona assembly on metazoan kinetochores. | |||
Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores.,Raisch T, Ciossani G, d'Amico E, Cmentowski V, Carmignani S, Maffini S, Merino F, Wohlgemuth S, Vetter IR, Raunser S, Musacchio A EMBO J. 2022 May 2;41(9):e110411. doi: 10.15252/embj.2021110411. Epub 2022 Apr 4. PMID:35373361<ref>PMID:35373361</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7qpg" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Carmignani S]] | |||
[[Category: Ciossani G]] | |||
[[Category: Cmetowski V]] | |||
[[Category: Maffini S]] | |||
[[Category: Merino F]] | |||
[[Category: Musacchio A]] | |||
[[Category: Raisch T]] | |||
[[Category: Raunser S]] | |||
[[Category: Vetter IR]] | |||
[[Category: Wohlgemuth S]] | |||
[[Category: D'Amico E]] | |||
Latest revision as of 12:38, 17 July 2024
Human RZZ kinetochore corona complex.
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