7uci: Difference between revisions
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New page: '''Unreleased structure''' The entry 7uci is ON HOLD Authors: Lao, Y., Skiba, M.A., Smith, J.L. Description: SxtA Methyltransferase and decarboxylase di-domain in complex with Mn2+ and... |
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==SxtA Methyltransferase and decarboxylase didomain in complex with Mn2+ and SAH== | |||
<StructureSection load='7uci' size='340' side='right'caption='[[7uci]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7uci]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cylindrospermopsis_raciborskii Cylindrospermopsis raciborskii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uci OCA], [https://pdbe.org/7uci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uci RCSB], [https://www.ebi.ac.uk/pdbsum/7uci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uci ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B3EYF9_9CYAN B3EYF9_9CYAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Installation of methyl groups can significantly improve the binding of small-molecule drugs to protein targets; however, site-selective methylation often presents a significant synthetic challenge. Metal- and S-adenosyl-methionine (SAM)-dependent methyltransferases (MTs) in natural-product biosynthetic pathways are powerful enzymatic tools for selective or chemically challenging C-methylation reactions. Each of these MTs selectively catalyzes one or two methyl transfer reactions. Crystal structures and biochemical assays of the Mn(2+)-dependent monomethyltransferase from the saxitoxin biosynthetic pathway (SxtA MT) revealed the structural basis for control of methylation extent. The SxtA monomethyltransferase was converted to a dimethyltransferase by modification of the metal binding site, addition of an active site base, and an amino acid substitution to provide space in the substrate pocket for two methyl substituents. A reciprocal change converted a related dimethyltransferase into a monomethyltransferase, supporting our hypothesis that steric hindrance can prevent a second methylation event. A novel understanding of MTs will accelerate the development of MT-based catalysts and MT engineering for use in small-molecule synthesis. | |||
Structural Basis for Control of Methylation Extent in Polyketide Synthase Metal-Dependent C-Methyltransferases.,Lao Y, Skiba MA, Chun SW, Narayan ARH, Smith JL ACS Chem Biol. 2022 Aug 19;17(8):2088-2098. doi: 10.1021/acschembio.2c00085. Epub, 2022 May 20. PMID:35594521<ref>PMID:35594521</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Lao | <div class="pdbe-citations 7uci" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cylindrospermopsis raciborskii]] | |||
[[Category: Large Structures]] | |||
[[Category: Lao Y]] | |||
[[Category: Skiba MA]] | |||
[[Category: Smith JL]] | |||
Latest revision as of 17:13, 18 October 2023
SxtA Methyltransferase and decarboxylase didomain in complex with Mn2+ and SAH
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