7qb9: Difference between revisions

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<StructureSection load='7qb9' size='340' side='right'caption='[[7qb9]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='7qb9' size='340' side='right'caption='[[7qb9]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7qb9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QB9 FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QB9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7qa8|7qa8]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qb9 OCA], [https://pdbe.org/7qb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qb9 RCSB], [https://www.ebi.ac.uk/pdbsum/7qb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qb9 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qb9 OCA], [https://pdbe.org/7qb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qb9 RCSB], [https://www.ebi.ac.uk/pdbsum/7qb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qb9 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The fungal class D1 G-protein-coupled receptor (GPCR) Ste2 has a different arrangement of transmembrane helices compared with mammalian GPCRs and a distinct mode of coupling to the heterotrimeric G protein Gpa1-Ste2-Ste18(1). In addition, Ste2 lacks conserved sequence motifs such as DRY, PIF and NPXXY, which are associated with the activation of class A GPCRs(2). This suggested that the activation mechanism of Ste2 may also differ. Here we determined structures of Saccharomyces cerevisiae Ste2 in the absence of G protein in two different conformations bound to the native agonist alpha-factor, bound to an antagonist and without ligand. These structures revealed that Ste2 is indeed activated differently from other GPCRs. In the inactive state, the cytoplasmic end of transmembrane helix H7 is unstructured and packs between helices H1-H6, blocking the G protein coupling site. Agonist binding results in the outward movement of the extracellular ends of H6 and H7 by 6 A. On the intracellular surface, the G protein coupling site is formed by a 20 A outward movement of the unstructured region in H7 that unblocks the site, and a 12 A inward movement of H6. This is a distinct mechanism in GPCRs, in which the movement of H6 and H7 upon agonist binding facilitates G protein coupling.
Activation mechanism of the class D fungal GPCR dimer Ste2.,Velazhahan V, Ma N, Vaidehi N, Tate CG Nature. 2022 Mar;603(7902):743-748. doi: 10.1038/s41586-022-04498-3. Epub 2022, Mar 16. PMID:35296853<ref>PMID:35296853</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qb9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Tate, C G]]
[[Category: Tate CG]]
[[Category: Velazhahan, V]]
[[Category: Velazhahan V]]
[[Category: Dimer]]
[[Category: Fungal]]
[[Category: Gpcr]]
[[Category: Ligand-free]]
[[Category: Membrane protein]]

Latest revision as of 13:55, 6 November 2024

Structure of the ligand-free GPCR dimer Ste2

7qb9, resolution 3.10Å

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