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New page: left|200px<br /> <applet load="1esl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1esl, resolution 2.0Å" /> '''INSIGHT INTO E-SELEC...
 
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[[Image:1esl.gif|left|200px]]<br />
<applet load="1esl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1esl, resolution 2.0&Aring;" />
'''INSIGHT INTO E-SELECTIN(SLASH)LIGAND INTERACTION FROM THE CRYSTAL STRUCTURE AND MUTAGENESIS OF THE LEC(SLASH)EGF DOMAINS'''<br />


==Overview==
==INSIGHT INTO E-SELECTIN(SLASH)LIGAND INTERACTION FROM THE CRYSTAL STRUCTURE AND MUTAGENESIS OF THE LEC(SLASH)EGF DOMAINS==
The three-dimensional structure of the ligand-binding region of human, E-selectin has been determined at 2.0 A resolution. The structure reveals, limited contact between the two domains and a coordination of Ca2+ not, predicted from other C-type lectins. Structure/function analysis indicates, a defined region and specific amino-acid side chains that may be involved, in ligand binding. These features of the E-selectin/ligand interaction, have important implications for understanding the recruitment of, leukocytes to sites of inflammation.
<StructureSection load='1esl' size='340' side='right'caption='[[1esl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1esl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esl OCA], [https://pdbe.org/1esl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esl RCSB], [https://www.ebi.ac.uk/pdbsum/1esl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LYAM2_HUMAN LYAM2_HUMAN] Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.<ref>PMID:1689848</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/1esl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1esl ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional structure of the ligand-binding region of human E-selectin has been determined at 2.0 A resolution. The structure reveals limited contact between the two domains and a coordination of Ca2+ not predicted from other C-type lectins. Structure/function analysis indicates a defined region and specific amino-acid side chains that may be involved in ligand binding. These features of the E-selectin/ligand interaction have important implications for understanding the recruitment of leukocytes to sites of inflammation.


==Disease==
Insight into E-selectin/ligand interaction from the crystal structure and mutagenesis of the lec/EGF domains.,Graves BJ, Crowther RL, Chandran C, Rumberger JM, Li S, Huang KS, Presky DH, Familletti PC, Wolitzky BA, Burns DK Nature. 1994 Feb 10;367(6463):532-8. PMID:7509040<ref>PMID:7509040</ref>
Known diseases associated with this structure: Atherosclerosis, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131210 131210]], Blood pressure regulation QTL OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131210 131210]], IgA nephropathy, susceptiblity to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131210 131210]]


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1ESL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ESL OCA].
</div>
<div class="pdbe-citations 1esl" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Insight into E-selectin/ligand interaction from the crystal structure and mutagenesis of the lec/EGF domains., Graves BJ, Crowther RL, Chandran C, Rumberger JM, Li S, Huang KS, Presky DH, Familletti PC, Wolitzky BA, Burns DK, Nature. 1994 Feb 10;367(6463):532-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7509040 7509040]
*[[Selectin|Selectin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Crowther, R.L.]]
[[Category: Crowther RL]]
[[Category: Graves, B.J.]]
[[Category: Graves BJ]]
[[Category: CA]]
[[Category: CL]]
[[Category: cell adhesion protein]]
 
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Retrieved from "https://proteopedia.org/1esl"