1h5d: Difference between revisions

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[[Image:1h5d.jpg|left|200px]]
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{{STRUCTURE_1h5d|  PDB=1h5d  |  SCENE=  }}
'''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (0-11% DOSE)'''


==X-ray induced reduction of horseradish peroxidase C1A Compound III (0-11% dose)==
<StructureSection load='1h5d' size='340' side='right'caption='[[1h5d]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1h5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H5D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h5d OCA], [https://pdbe.org/1h5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h5d RCSB], [https://www.ebi.ac.uk/pdbsum/1h5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h5d ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h5/1h5d_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h5d ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.


==Overview==
The catalytic pathway of horseradish peroxidase at high resolution.,Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J Nature. 2002 May 23;417(6887):463-8. PMID:12024218<ref>PMID:12024218</ref>
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1H5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5D OCA].
</div>
<div class="pdbe-citations 1h5d" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12024218 12024218]
*[[Horseradish peroxidase|Horseradish peroxidase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Armoracia rusticana]]
[[Category: Armoracia rusticana]]
[[Category: Peroxidase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Berglund GI]]
[[Category: Berglund, G I.]]
[[Category: Carlsson GH]]
[[Category: Carlsson, G H.]]
[[Category: Hajdu J]]
[[Category: Hajdu, J.]]
[[Category: Henriksen A]]
[[Category: Henriksen, A.]]
[[Category: Smith AT]]
[[Category: Smith, A T.]]
[[Category: Szoke H]]
[[Category: Szoke, H.]]
[[Category: Compound iii]]
[[Category: Horseradish]]
[[Category: Oxidoreductase]]
[[Category: Oxyperoxidase]]
[[Category: Peroxidase]]
[[Category: X-ray induced reduction]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:27:11 2008''
Retrieved from "https://proteopedia.org/1h5d"