7r0j: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Structure of the V2 receptor Cter-arrestin2-ScFv30 complex== | |||
<StructureSection load='7r0j' size='340' side='right'caption='[[7r0j]], [[Resolution|resolution]] 4.23Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7r0j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R0J FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.23Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r0j OCA], [https://pdbe.org/7r0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r0j RCSB], [https://www.ebi.ac.uk/pdbsum/7r0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r0j ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Arrestins interact with G protein-coupled receptors (GPCRs) to stop G protein activation and to initiate key signaling pathways. Recent structural studies shed light on the molecular mechanisms involved in GPCR-arrestin coupling, but whether this process is conserved among GPCRs is poorly understood. Here, we report the cryo-electron microscopy active structure of the wild-type arginine-vasopressin V2 receptor (V2R) in complex with beta-arrestin1. It reveals an atypical position of beta-arrestin1 compared to previously described GPCR-arrestin assemblies, associated with an original V2R/beta-arrestin1 interface involving all receptor intracellular loops. Phosphorylated sites of the V2R carboxyl terminus are clearly identified and interact extensively with the beta-arrestin1 N-lobe, in agreement with structural data obtained with chimeric or synthetic systems. Overall, these findings highlight a notable structural variability among GPCR-arrestin signaling complexes. | |||
Structure of the vasopressin hormone-V2 receptor-beta-arrestin1 ternary complex.,Bous J, Fouillen A, Orcel H, Trapani S, Cong X, Fontanel S, Saint-Paul J, Lai-Kee-Him J, Urbach S, Sibille N, Sounier R, Granier S, Mouillac B, Bron P Sci Adv. 2022 Sep 2;8(35):eabo7761. doi: 10.1126/sciadv.abo7761. Epub 2022 Sep 2. PMID:36054364<ref>PMID:36054364</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Bous | <div class="pdbe-citations 7r0j" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Fouillen | ==See Also== | ||
[[Category: | *[[Arrestin 3D structures|Arrestin 3D structures]] | ||
[[Category: Mouillac | == References == | ||
[[Category: Trapani | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Bous J]] | |||
[[Category: Bron P]] | |||
[[Category: Fouillen A]] | |||
[[Category: Granier S]] | |||
[[Category: Mouillac B]] | |||
[[Category: Trapani S]] | |||
