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New page: left|200px<br /> <applet load="1f2h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f2h" /> '''SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN...
 
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[[Image:1f2h.gif|left|200px]]<br />
<applet load="1f2h" size="450" color="white" frame="true" align="right" spinBox="true"
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'''SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TNFR1 ASSOCIATED PROTEIN, TRADD.'''<br />


==Overview==
==SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TNFR1 ASSOCIATED PROTEIN, TRADD.==
TRADD is a multifunctional signaling adaptor protein that is recruited to, TNFR1 upon ligand binding. The C-terminal of TRADD comprises the "death, domain" that is responsible for association of TNFR1 and other death, domain-containing proteins such as FADD and RIP. The N-terminal domain, (N-TRADD) promotes the recruitment of TRAF2 to TNFR1 by binding to the, C-terminal of TRAF2, leading to the activation of JNK/AP1 and NF-kappa B., The solution structure of N-TRADD was determined, revealing a novel, protein fold. A combination of NMR, BIAcore, and mutagenesis experiments, was used to help identify the site of interaction of N-TRADD with C-TRAF2, providing a framework for future attempts to selectively inhibit the TNF, signaling pathways.
<StructureSection load='1f2h' size='340' side='right'caption='[[1f2h]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1f2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F2H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2h OCA], [https://pdbe.org/1f2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f2h RCSB], [https://www.ebi.ac.uk/pdbsum/1f2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRADD_HUMAN TRADD_HUMAN] The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/1f2h_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f2h ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
TRADD is a multifunctional signaling adaptor protein that is recruited to TNFR1 upon ligand binding. The C-terminal of TRADD comprises the "death domain" that is responsible for association of TNFR1 and other death domain-containing proteins such as FADD and RIP. The N-terminal domain (N-TRADD) promotes the recruitment of TRAF2 to TNFR1 by binding to the C-terminal of TRAF2, leading to the activation of JNK/AP1 and NF-kappa B. The solution structure of N-TRADD was determined, revealing a novel protein fold. A combination of NMR, BIAcore, and mutagenesis experiments was used to help identify the site of interaction of N-TRADD with C-TRAF2, providing a framework for future attempts to selectively inhibit the TNF signaling pathways.


==About this Structure==
Solution structure of N-TRADD and characterization of the interaction of N-TRADD and C-TRAF2, a key step in the TNFR1 signaling pathway.,Tsao DH, McDonagh T, Telliez JB, Hsu S, Malakian K, Xu GY, Lin LL Mol Cell. 2000 Jun;5(6):1051-7. PMID:10911999<ref>PMID:10911999</ref>
1F2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F2H OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Solution structure of N-TRADD and characterization of the interaction of N-TRADD and C-TRAF2, a key step in the TNFR1 signaling pathway., Tsao DH, McDonagh T, Telliez JB, Hsu S, Malakian K, Xu GY, Lin LL, Mol Cell. 2000 Jun;5(6):1051-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10911999 10911999]
</div>
<div class="pdbe-citations 1f2h" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Hsu, H.]]
[[Category: Hsu H]]
[[Category: Lin, L.L.]]
[[Category: Lin L-L]]
[[Category: Malakian, K.]]
[[Category: Malakian K]]
[[Category: McDonaugh, T.]]
[[Category: McDonaugh T]]
[[Category: Telliez, J.B.]]
[[Category: Telliez J-B]]
[[Category: Tsao, D.]]
[[Category: Tsao D]]
[[Category: Xu, G.Y.]]
[[Category: Xu G-Y]]
[[Category: tnfr-1 associated protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:48:31 2007''

Latest revision as of 08:27, 22 May 2024

SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TNFR1 ASSOCIATED PROTEIN, TRADD.

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