3qac: Difference between revisions
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<StructureSection load='3qac' size='340' side='right'caption='[[3qac]], [[Resolution|resolution]] 2.27Å' scene=''> | <StructureSection load='3qac' size='340' side='right'caption='[[3qac]], [[Resolution|resolution]] 2.27Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3qac]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3qac]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QAC FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.275Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qac OCA], [https://pdbe.org/3qac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qac RCSB], [https://www.ebi.ac.uk/pdbsum/3qac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qac ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qac OCA], [https://pdbe.org/3qac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qac RCSB], [https://www.ebi.ac.uk/pdbsum/3qac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qac ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q38712_AMAHP Q38712_AMAHP] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress. | 11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress. | ||
Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.,Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):919-22. Epub 2010 Jul 29. PMID: | Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.,Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):919-22. Epub 2010 Jul 29. PMID:020693668<ref>PMID:020693668</ref> | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Amaranthus hypochondriacus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Carrazco-Pena | [[Category: Barba de la Rosa AP]] | ||
[[Category: Maruyama | [[Category: Carrazco-Pena L]] | ||
[[Category: Mikami | [[Category: Maruyama N]] | ||
[[Category: Osuna-Castro | [[Category: Mikami B]] | ||
[[Category: Osuna-Castro JA]] | |||
[[Category: Tandang-Silvas | [[Category: Tandang-Silvas MR]] | ||
[[Category: Utsumi | [[Category: Utsumi S]] | ||
