8a2q: Difference between revisions
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==Structure of the DNA-bound FANCD2-FANCI complex containing phosphomimetic FANCI== | |||
<StructureSection load='8a2q' size='340' side='right'caption='[[8a2q]], [[Resolution|resolution]] 3.53Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8a2q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A2Q FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.53Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a2q OCA], [https://pdbe.org/8a2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a2q RCSB], [https://www.ebi.ac.uk/pdbsum/8a2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a2q ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B0I564_CHICK B0I564_CHICK] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
DNA interstrand cross-links are tumor-inducing lesions that block DNA replication and transcription. When cross-links are detected at stalled replication forks, ATR kinase phosphorylates FANCI, which stimulates monoubiquitination of the FANCD2-FANCI clamp by the Fanconi anemia core complex. Monoubiquitinated FANCD2-FANCI is locked onto DNA and recruits nucleases that mediate DNA repair. However, it remains unclear how phosphorylation activates this pathway. Here, we report structures of FANCD2-FANCI complexes containing phosphomimetic FANCI. We observe that, unlike wild-type FANCD2-FANCI, the phosphomimetic complex closes around DNA, independent of the Fanconi anemia core complex. The phosphomimetic mutations do not substantially alter DNA binding but instead destabilize the open state of FANCD2-FANCI and alter its conformational dynamics. Overall, our results demonstrate that phosphorylation primes the FANCD2-FANCI clamp for ubiquitination, showing how multiple posttranslational modifications are coordinated to control DNA repair. | |||
The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination.,Sijacki T, Alcon P, Chen ZA, McLaughlin SH, Shakeel S, Rappsilber J, Passmore LA Nat Struct Mol Biol. 2022 Sep;29(9):881-890. doi: 10.1038/s41594-022-00820-9. , Epub 2022 Sep 1. PMID:36050501<ref>PMID:36050501</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8a2q" style="background-color:#fffaf0;"></div> | ||
[[Category: Alcon | == References == | ||
[[Category: Sijacki | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Gallus gallus]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Alcon P]] | |||
[[Category: Passmore LA]] | |||
[[Category: Sijacki T]] | |||
Latest revision as of 06:43, 24 July 2024
Structure of the DNA-bound FANCD2-FANCI complex containing phosphomimetic FANCI
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