8d9f: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: '''Unreleased structure''' The entry 8d9f is ON HOLD Authors: Description: Category: Unreleased Structures
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 8d9f is ON HOLD
==gRAMP-TPR-CHAT (Craspase)==
<StructureSection load='8d9f' size='340' side='right'caption='[[8d9f]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8d9f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D9F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.71&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d9f OCA], [https://pdbe.org/8d9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d9f RCSB], [https://www.ebi.ac.uk/pdbsum/8d9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d9f ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The CRISPR-Cas type III-E RNA-targeting effector complex gRAMP/Cas7-11 is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here, we use cryo-electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5' region of the guide RNA displaces a gating loop in gRAMP, which triggers an extensive conformational relay that allosterically aligns the protease catalytic dyad and opens an amino acid side-chain-binding pocket. We further define Csx30 as the endogenous protein substrate that is site-specifically proteolyzed by RNA-activated Craspase. This protease activity is switched off by target RNA cleavage by gRAMP and is not activated by RNA targets containing a matching protospacer flanking sequence. We thus conclude that Craspase is a target RNA-activated protease with self-regulatory capacity.


Authors:  
Craspase is a CRISPR RNA-guided, RNA-activated protease.,Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodriguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A Science. 2022 Sep 16;377(6612):1278-1285. doi: 10.1126/science.add5064. Epub 2022 , Aug 25. PMID:36007061<ref>PMID:36007061</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 8d9f" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Candidatus Scalindua brodae]]
[[Category: Large Structures]]
[[Category: Hu C]]
[[Category: Ke A]]
[[Category: Nam KH]]
[[Category: Schuler G]]
Retrieved from "https://proteopedia.org/8d9f"