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| | ==THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME== |
| The line below this paragraph, containing "STRUCTURE_1hmh", creates the "Structure Box" on the page.
| | <StructureSection load='1hmh' size='340' side='right'caption='[[1hmh]], [[Resolution|resolution]] 2.60Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1hmh]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HMH FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| --> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hmh OCA], [https://pdbe.org/1hmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hmh RCSB], [https://www.ebi.ac.uk/pdbsum/1hmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hmh ProSAT]</span></td></tr> |
| {{STRUCTURE_1hmh| PDB=1hmh | SCENE= }}
| | </table> |
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| '''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME'''
| | ==See Also== |
| | | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.
| | [[Category: Large Structures]] |
| | | [[Category: Flaherty KM]] |
| ==About this Structure==
| | [[Category: McKay DB]] |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA].
| | [[Category: Pley HW]] |
| | |
| ==Reference==
| |
| Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422]
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| [[Category: Flaherty, K M.]] | |
| [[Category: McKay, D B.]] | |
| [[Category: Pley, H W.]] | |
| [[Category: Dna-rna hammerhead ribozyme]] | |
| [[Category: Loop]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:00:55 2008''
| |
