7yev: Difference between revisions
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New page: '''Unreleased structure''' The entry 7yev is ON HOLD Authors: Bao, K.Y., Zhang, X.L., Li, D.Y., Zhu, P. Description: In situ structure of polymerase complex of mammalian reovirus in th... |
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The | ==In situ structure of polymerase complex of mammalian reovirus in the pre-elongation state== | ||
<StructureSection load='7yev' size='340' side='right'caption='[[7yev]], [[Resolution|resolution]] 3.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7yev]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YEV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yev OCA], [https://pdbe.org/7yev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yev RCSB], [https://www.ebi.ac.uk/pdbsum/7yev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yev ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/C9E874_9REOV C9E874_9REOV] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mammalian reovirus (reovirus) is a multilayered, turreted member of Reoviridae characterized by transcription of dsRNA genome within the innermost capsid shell. Here, we present high-resolution in situ structures of reovirus transcriptase complex in an intact double-layered virion, and in the uncoated single-layered core particles in the unloaded, reloaded, pre-elongation, and elongation states, respectively, obtained by cryo-electron microscopy and sub-particle reconstructions. At the template entry of RNA-dependent RNA polymerase (RdRp), the RNA-loading region gets flexible after uncoating resulting in the unloading of terminal genomic RNA and inactivity of transcription. However, upon adding transcriptional substrates, the RNA-loading region is recovered leading the RNAs loaded again. The priming loop in RdRp was found to play a critical role in regulating transcription, which hinders the elongation of transcript in virion and triggers the rearrangement of RdRp C-terminal domain (CTD) during elongation, resulting in splitting of template-transcript hybrid and opening of transcript exit. With the integration of these structures, a transcriptional model of reovirus with five states is proposed. Our structures illuminate the RdRp activation and regulation of the multilayered turreted reovirus. | |||
In situ structures of polymerase complex of mammalian reovirus illuminate RdRp activation and transcription regulation.,Bao K, Zhang X, Li D, Sun W, Sun Z, Wang J, Zhu P Proc Natl Acad Sci U S A. 2022 Dec 13;119(50):e2203054119. doi: , 10.1073/pnas.2203054119. Epub 2022 Dec 5. PMID:36469786<ref>PMID:36469786</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Bao | <div class="pdbe-citations 7yev" style="background-color:#fffaf0;"></div> | ||
[[Category: Li | == References == | ||
[[Category: Zhang | <references/> | ||
[[Category: Zhu | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mammalian orthoreovirus 3]] | |||
[[Category: Bao KY]] | |||
[[Category: Li DY]] | |||
[[Category: Zhang XL]] | |||
[[Category: Zhu P]] | |||
Latest revision as of 05:59, 17 September 2025
In situ structure of polymerase complex of mammalian reovirus in the pre-elongation state
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